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验证数据展示

  • WB analysis using 33482-1-AP (same clone as 33482-1-PBS)

    WB analysis using 33482-1-AP (same clone as 33482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33482-1-AP (POLE2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33482-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 33482-1-AP (same clone as 33482-1-PBS)

    IF Staining of A431 using 33482-1-AP (same clone as 33482-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using POLE2 antibody (33482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 33482-1-PBS in a different storage buffer formulation.

POLE2 Polyclonal antibody, PBS Only

POLE2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 33482-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

DNA polymerase epsilon subunit 2, DNA polymerase epsilon subunit B, DNA polymerase II subunit 2, DPE2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

33482-1-PBS targets POLE2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag38070

Product name: Recombinant human POLE2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 178-527 aa of BC126218

Sequence: IGDAIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHVNAFGFPPTEPSSTTRAYYGNINFFGGPSNTSVKTSAKLKQLEEENKDAMFVFLSDVWLDQVEVLEKLRIMFAGYSPAPPTCFILCGNFSSAPYGKNQVQALKDSLKTLADIICEYPDIHQSSRFVFVPGPEDPGFGSILPRPPLAESITNEFRQRVPFSVFTTNPCRIQYCTQEITVFREDLVNKMCRNCVRFPSSNLAIPNHFVKTILSQGHLTPLPLYVCPVYWAYDYALRVYPVPDLLVIADKYDPFTTTNTECLCINPGSFPRSGFSFKVFYPSNKTVEDSKLQGF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (DNA directed), epsilon 2 (p59 subunit)
别名 DNA polymerase epsilon subunit 2, DNA polymerase epsilon subunit B, DNA polymerase II subunit 2, DPE2
计算分子量 527 aa, 60 kDa
观测分子量60 kDa
GenBank蛋白编号BC126218
基因名称 POLE2
Gene ID (NCBI) 5427
RRIDAB_3742987
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP56282
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNA polymerase epsilon subunit 2 (POLE2) is involved in cellular functions such as DNA replication, repair, and cell cycle control, as well as in the array-based proliferative signatures (PMID: 9745046; 12620412). Knockdown of POLE2 could suppress esophageal squamous cell carcinoma development through regulating cell proliferation, apoptosis, and migration (PMID: 32831648). POLE2 can also regulate the development of oral squamous cell carcinoma by inhibiting the PI3K/AKT signaling pathway (PMID: 38079056).

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