验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, MDA-MB-231 cells |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33482-1-AP targets POLE2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38070 Product name: Recombinant human POLE2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 178-527 aa of BC126218 Sequence: IGDAIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHVNAFGFPPTEPSSTTRAYYGNINFFGGPSNTSVKTSAKLKQLEEENKDAMFVFLSDVWLDQVEVLEKLRIMFAGYSPAPPTCFILCGNFSSAPYGKNQVQALKDSLKTLADIICEYPDIHQSSRFVFVPGPEDPGFGSILPRPPLAESITNEFRQRVPFSVFTTNPCRIQYCTQEITVFREDLVNKMCRNCVRFPSSNLAIPNHFVKTILSQGHLTPLPLYVCPVYWAYDYALRVYPVPDLLVIADKYDPFTTTNTECLCINPGSFPRSGFSFKVFYPSNKTVEDSKLQGF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | polymerase (DNA directed), epsilon 2 (p59 subunit) |
| 别名 | DNA polymerase epsilon subunit 2, DNA polymerase epsilon subunit B, DNA polymerase II subunit 2, DPE2 |
| 计算分子量 | 527 aa, 60 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC126218 |
| 基因名称 | POLE2 |
| Gene ID (NCBI) | 5427 |
| RRID | AB_3742987 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P56282 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNA polymerase epsilon subunit 2 (POLE2) is involved in cellular functions such as DNA replication, repair, and cell cycle control, as well as in the array-based proliferative signatures (PMID: 9745046; 12620412). Knockdown of POLE2 could suppress esophageal squamous cell carcinoma development through regulating cell proliferation, apoptosis, and migration (PMID: 32831648). POLE2 can also regulate the development of oral squamous cell carcinoma by inhibiting the PI3K/AKT signaling pathway (PMID: 38079056).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for POLE2 antibody 33482-1-AP | Download protocol |
| WB protocol for POLE2 antibody 33482-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
