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验证数据展示

  • WB analysis using 86232-3-RR (same clone as 86232-3-PBS)

    WB analysis using 86232-3-RR (same clone as 86232-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86232-3-RR (POLDIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86232-3-PBS in a different storage buffer formulation.

  • WB analysis of mouse testis using 86232-3-RR (same clone as 86232-3-PBS)

    WB analysis of mouse testis using 86232-3-RR (same clone as 86232-3-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 86232-3-RR (POLDIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86232-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86232-3-RR (same clone as 86232-3-PBS)

    IF Staining of HeLa using 86232-3-RR (same clone as 86232-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLDIP2 antibody (86232-3-RR, Clone: 250999B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86232-3-PBS in a different storage buffer formulation.

POLDIP2 Recombinant monoclonal antibody, PBS Only

POLDIP2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86232-3-PBS

产品说明书

CloneNo. 250999B11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

HSPC017, p38, PDIP38, POLD4, Polymerase delta-interacting protein 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® POLDIP2 Ready-To-Use IHC Kit. POLDIP2 primary antibody included.

产品信息

86232-3-PBS targets POLDIP2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7154

Product name: Recombinant human POLDIP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-368 aa of BC000655

Sequence: MAACTARRALAVGSRWWSRSLTGARWPRPLCAAAGAGAFSPASTTTTRRHLSSRNRPEGKVLETVGVFEVPKQNGKYETGQLFLHSIFGYRGVVLFPWQARLYDRDVASAAPEKAENPAGHGSKEVKGKTHTYYQVLIDARDCPHISQRSQTEAVTFLANHDDSRALYAIPGLDYVSHEDILPYTSTDQVPIQHELFERFLLYDQTKAPPFVARETLRAWQEKNHPWLELSDVHRETTENIRVTVIPFYMGMREAQNSHVYWWRYCIRLENLDSDVVQLRERHWRIFSLSGTLETVRGRGVVGREPVLSKEQPAFQYSSHVSLQASSGHMWGTFRFERPDGSHFDVRIPPFSLESNKDEKTPPSGLHW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 polymerase (DNA-directed), delta interacting protein 2
别名 HSPC017, p38, PDIP38, POLD4, Polymerase delta-interacting protein 2
计算分子量 42 kDa
观测分子量37 kDa
GenBank蛋白编号BC000655
基因名称 POLDIP2
Gene ID (NCBI) 26073
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y2S7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

POLDIP2(Polymerase delta-interacting protein 2) is also named as PDIP38, POLD4. It is as a unique positive regulator of Nox4 via its association with p22phox and together with Nox4, has profound effects on Rhodependent cytoskeletal remodeling. It is also as a regulator of cell division. It consists of 368 amino acids and has a predicted molecular mass of 42 kDa, with a potential signal peptide cleavage site after the first N-terminal 48 residues, which would result in a protein of 37 kDa(PMID:19574552). Endogenous human and rat POLDIP2 localized to the nucleus, plasma membrane, and cytoplasm(PMID:17623671).

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