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验证数据展示

  • WB analysis using 30106-1-AP

    WB analysis using 30106-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30106-1-AP (POGZ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 30106-1-AP

    IHC staining of mouse brain using 30106-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with POGZ antibody included
  • IF Staining of HepG2 using 30106-1-AP

    IF Staining of HepG2 using 30106-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POGZ antibody (30106-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IP experiment of HeLa using 30106-1-AP

    IP experiment of HeLa using 30106-1-AP

    IP result of anti-POGZ (IP:30106-1-AP, 4ug; Detection:30106-1-AP 1:2000) with HeLa cells lysate 1800 ug.

  • IHC staining of mouse testis using 30106-1-AP

    IHC staining of mouse testis using 30106-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with POGZ antibody included
  • IHC staining of mouse testis using 30106-1-AP

    IHC staining of mouse testis using 30106-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with POGZ antibody included
  • IHC staining of mouse brain using 30106-1-AP

    IHC staining of mouse brain using 30106-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with POGZ antibody included
  • IF Staining of U-251 using 30106-1-AP

    IF Staining of U-251 using 30106-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using POGZ antibody (30106-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

POGZ Polyclonal antibody

POGZ Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 30106-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

KIAA0461, POGZ, SUHW5, ZNF280E, ZNF635, ZNF635m

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, SH-SY5Y cells, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, U-251 cells
Planning an IHC experiment? We recommend our IHCeasy POGZ Ready-To-Use IHC Kit. POGZ primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30106-1-AP targets POGZ in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag32435

Product name: Recombinant human POGZ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1178-1400 aa of NM_015100

Sequence: GQMDQPANMPDSILLEAKESGYSDDEIMELWSTRVWQKHTACQRSKGMLVMDCHRTHLSEEVLAMLSASSTLPAVVPAGCSSKIQPLDVCIKRTVKNFLHKKWKEQAREMADTACDSDVLLQLVLVWLGEVLGVIGDCPELVQRSFLVASVLPGPDGNINSPTRNADMQEELIASLEEQLKLSGEHSESSTPRPRSSPEETIEPESLHQLFEGESETESFYGFEEADLDLMEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pogo transposable element with ZNF domain
别名 KIAA0461, POGZ, SUHW5, ZNF280E, ZNF635, ZNF635m
计算分子量155kd
观测分子量 155 kDa
GenBank蛋白编号NM_015100
基因名称 POGZ
Gene ID (NCBI) 23126
RRIDAB_2935514
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z3K3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POGZ, also known as MRD37, is a zinc-finger protein containing 9 C2H2 zinc fingers, a CENP-B (centromere protein-B) domain, and a DDE domain found in the bacterial transposase/retroviral integrase family. POGZ plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. POGZ plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms (PMID:20562864). Heterozygous mutation of POGZ causes White-Sutton syndrome (PMID: 26739615). Expect a band approximately 155 kDa in size corresponding to POGZ by western blotting.

实验方案

Product Specific Protocols
WB protocol for POGZ antibody 30106-1-APDownload protocol
IHC protocol for POGZ antibody 30106-1-APDownload protocol
IF protocol for POGZ antibody 30106-1-APDownload protocol
IP protocol for POGZ antibody 30106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols