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POGZ Polyclonal antibody

POGZ Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 30106-1-AP

Print datasheet

Synonyms

KIAA0461, POGZ, SUHW5, ZNF280E, ZNF635, ZNF635m

验证数据展示

  • WB analysis using 30106-1-AP

    WB analysis using 30106-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30106-1-AP (POGZ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 30106-1-AP

    IHC staining of mouse brain using 30106-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with POGZ antibody included
  • IF Staining of HepG2 using 30106-1-AP

    IF Staining of HepG2 using 30106-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POGZ antibody (30106-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IP experiment of HeLa using 30106-1-AP

    IP experiment of HeLa using 30106-1-AP

    IP result of anti-POGZ (IP:30106-1-AP, 4ug; Detection:30106-1-AP 1:2000) with HeLa cells lysate 1800 ug.

  • IHC staining of mouse testis using 30106-1-AP

    IHC staining of mouse testis using 30106-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with POGZ antibody included
  • IHC staining of mouse testis using 30106-1-AP

    IHC staining of mouse testis using 30106-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with POGZ antibody included
  • IHC staining of mouse brain using 30106-1-AP

    IHC staining of mouse brain using 30106-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with POGZ antibody included
  • IF Staining of U-251 using 30106-1-AP

    IF Staining of U-251 using 30106-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using POGZ antibody (30106-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, SH-SY5Y cells, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, U-251 cells
Planning an IHC experiment? We recommend our IHCeasy POGZ Ready-To-Use IHC Kit. POGZ primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30106-1-AP targets POGZ in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity Human, mouse
Immunogen POGZ fusion protein Ag32435 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pogo transposable element with ZNF domain
Synonyms KIAA0461, POGZ, SUHW5, ZNF280E, ZNF635, ZNF635m
Calculated Molecular Weight155kd
Observed Molecular Weight 155 kDa
GenBank Accession NumberNM_015100
Gene Symbol POGZ
Gene ID (NCBI) 23126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z3K3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POGZ, also known as MRD37, is a zinc-finger protein containing 9 C2H2 zinc fingers, a CENP-B (centromere protein-B) domain, and a DDE domain found in the bacterial transposase/retroviral integrase family. POGZ plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. POGZ plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms (PMID:20562864). Heterozygous mutation of POGZ causes White-Sutton syndrome (PMID: 26739615). Expect a band approximately 155 kDa in size corresponding to POGZ by western blotting.

实验方案

Product Specific Protocols
WB protocol for POGZ antibody 30106-1-APDownload protocol
IHC protocol for POGZ antibody 30106-1-APDownload protocol
IF protocol for POGZ antibody 30106-1-APDownload protocol
IP protocol for POGZ antibody 30106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols