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验证数据展示

  • WB analysis using 68309-1-Ig (same clone as 68309-1-PBS)

    WB analysis using 68309-1-Ig (same clone as 68309-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68309-1-Ig (PNPT1 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 68309-1-Ig (same clone as 68309-1-PBS)

    FC experiment of HepG2 using 68309-1-Ig (same clone as 68309-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.2 μg PNPT1 Monoclonal antibody (68309-1-Ig, Clone:3F2A1, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone:1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 68309-1-Ig (same clone as 68309-1-PBS)

    IHC staining of human stomach cancer using 68309-1-Ig (same clone as 68309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68309-1-Ig (PNPT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PNPT1 antibody included
  • IHC staining of human stomach cancer using 68309-1-Ig (same clone as 68309-1-PBS)

    IHC staining of human stomach cancer using 68309-1-Ig (same clone as 68309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68309-1-Ig (PNPT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PNPT1 antibody included
  • IHC staining of mouse stomach using 68309-1-Ig (same clone as 68309-1-PBS)

    IHC staining of mouse stomach using 68309-1-Ig (same clone as 68309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68309-1-Ig (PNPT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PNPT1 antibody included
  • IF Staining of HeLa using 68309-1-Ig (same clone as 68309-1-PBS)

    IF Staining of HeLa using 68309-1-Ig (same clone as 68309-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PNPT1 antibody (68309-1-Ig, Clone: 3F2A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

  • FC experiment of LNCaP using 68309-1-Ig (same clone as 68309-1-PBS)

    FC experiment of LNCaP using 68309-1-Ig (same clone as 68309-1-PBS)

    1x10^6 LNCaP cells were intracellularly stained with 0.2 μg PNPT1 Monoclonal antibody (68309-1-Ig, Clone:3F2A1, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone:1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

PNPT1 Monoclonal antibody, PBS Only

PNPT1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68309-1-PBS

产品说明书

CloneNo. 3F2A1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

3'-5' RNA exonuclease OLD35, 3F2A1, EC:2.7.7.8, old 35, OLD35

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PNPT1 Ready-To-Use IHC Kit. PNPT1 primary antibody included.

产品信息

68309-1-PBS targets PNPT1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PNPT1 fusion protein Ag6290 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 polyribonucleotide nucleotidyltransferase 1
别名 3'-5' RNA exonuclease OLD35, 3F2A1, EC:2.7.7.8, old 35, OLD35
计算分子量 86 kDa
观测分子量 86 kDa
GenBank蛋白编号BC053660
基因名称 PNPT1
Gene ID (NCBI) 87178
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8TCS8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.