验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, LNCap cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68309-1-Ig targets PNPT1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | Human, Mouse, Rat |
免疫原 | PNPT1 fusion protein Ag6290 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | polyribonucleotide nucleotidyltransferase 1 |
别名 | 3 5 RNA exonuclease OLD35, DKFZp762K1914, old 35, OLD35, PNPASE, PNPase 1, PNPase old 35, PNPT1, Polynucleotide phosphorylase 1 |
计算分子量 | 86 kDa |
观测分子量 | 86 kDa |
GenBank蛋白编号 | BC053660 |
基因名称 | PNPT1 |
Gene ID (NCBI) | 87178 |
RRID | AB_2935388 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q8TCS8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.
实验方案
Product Specific Protocols | |
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WB protocol for PNPT1 antibody 68309-1-Ig | Download protocol |
IF protocol for PNPT1 antibody 68309-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |