PNPT1 Monoclonal antibody
PNPT1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
3F2A1
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, LNCap cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68309-1-Ig targets PNPT1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | WB, ELISA |
Tested Reactivity | Human, Mouse, Rat |
Immunogen | PNPT1 fusion protein Ag6290 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | polyribonucleotide nucleotidyltransferase 1 |
Synonyms | 3 5 RNA exonuclease OLD35, DKFZp762K1914, old 35, OLD35, PNPASE, PNPase 1, PNPase old 35, PNPT1, Polynucleotide phosphorylase 1 |
Calculated Molecular Weight | 86 kDa |
Observed Molecular Weight | 86 kDa |
GenBank Accession Number | BC053660 |
Gene Symbol | PNPT1 |
Gene ID (NCBI) | 87178 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q8TCS8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.
实验方案
Product Specific Protocols | |
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WB protocol for PNPT1 antibody 68309-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |