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PNPLA4 Polyclonal antibody

PNPLA4 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 25469-1-AP

Print datasheet

Synonyms

DXS1283E, GS2, IPLA2 ETA, PNPLA4, Protein GS2

验证数据展示

  • WB analysis of HeLa using 25469-1-AP

    WB analysis of HeLa using 25469-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25469-1-AP (PNPLA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 25469-1-AP

    IP experiment of HeLa using 25469-1-AP

    IP result of anti-PNPLA4 (IP:25469-1-AP, 4ug; Detection:25469-1-AP 1:300) with HeLa cells lysate 3200ug.

  • IHC staining of human cervical cancer using 25469-1-AP

    IHC staining of human cervical cancer using 25469-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25469-1-AP (PNPLA4 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human cervical cancer using 25469-1-AP

    IHC staining of human cervical cancer using 25469-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25469-1-AP (PNPLA4 antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 25469-1-AP is PNPLA4 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen PNPLA4 fusion protein Ag22091 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name patatin-like phospholipase domain containing 4
Synonyms DXS1283E, GS2, IPLA2 ETA, PNPLA4, Protein GS2
Calculated Molecular Weight 253 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC020746
Gene Symbol PNPLA4
Gene ID (NCBI) 8228
RRIDAB_2880095
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP41247
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PNPLA4(Patatin-like phospholipase domain-containing protein 4) is also named as DXS1283E, GS2. It participate in triacylglycerol hydrolysis and the acyl-CoA independent transacylation of acylglycerols, there by facilitating energy mobilization and storage in adipocytes. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PNPLA4 antibody 25469-1-APDownload protocol
IHC protocol for PNPLA4 antibody 25469-1-APDownload protocol
IP protocol for PNPLA4 antibody 25469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Lysophosphatidylserine induces necrosis in pressure overloaded male mouse hearts via G protein coupled receptor 34

Authors - Ryuta Sugihara
View Article