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验证数据展示

  • WB analysis of HeLa using 25469-1-AP

    WB analysis of HeLa using 25469-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25469-1-AP (PNPLA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 25469-1-AP

    IP experiment of HeLa using 25469-1-AP

    IP result of anti-PNPLA4 (IP:25469-1-AP, 4ug; Detection:25469-1-AP 1:300) with HeLa cells lysate 3200ug.

  • IHC staining of human cervical cancer using 25469-1-AP

    IHC staining of human cervical cancer using 25469-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25469-1-AP (PNPLA4 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human cervical cancer using 25469-1-AP

    IHC staining of human cervical cancer using 25469-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25469-1-AP (PNPLA4 antibody) at dilution of 1:200 (under 40x lens).

PNPLA4 Polyclonal antibody

PNPLA4 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 25469-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

DXS1283E, GS2, IPLA2 ETA, PNPLA4, Protein GS2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25469-1-AP targets PNPLA4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 PNPLA4 fusion protein Ag22091 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 patatin-like phospholipase domain containing 4
别名 DXS1283E, GS2, IPLA2 ETA, PNPLA4, Protein GS2
计算分子量 253 aa, 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC020746
基因名称 PNPLA4
Gene ID (NCBI) 8228
RRIDAB_2880095
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP41247
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PNPLA4(Patatin-like phospholipase domain-containing protein 4) is also named as DXS1283E, GS2. It participate in triacylglycerol hydrolysis and the acyl-CoA independent transacylation of acylglycerols, there by facilitating energy mobilization and storage in adipocytes. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PNPLA4 antibody 25469-1-APDownload protocol
IHC protocol for PNPLA4 antibody 25469-1-APDownload protocol
IP protocol for PNPLA4 antibody 25469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Lysophosphatidylserine induces necrosis in pressure overloaded male mouse hearts via G protein coupled receptor 34

Authors - Ryuta Sugihara
View Article