PNPLA3 Monoclonal antibody
PNPLA3 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat, pig
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1D2B11
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells, SMMC-7721 cells, HuH-7 cells, HSC-T6 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67369-1-Ig targets PNPLA3 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Rat, pig samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Tested Reactivity | Human, Rat, pig |
| Immunogen | PNPLA3 fusion protein Ag27407 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | patatin-like phospholipase domain containing 3 |
| Synonyms | Acylglycerol O acyltransferase, Adiponutrin, ADPN, C22orf20, iPLA(2)epsilon, iPLA2 epsilon, PNPLA3 |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC065195 |
| Gene Symbol | PNPLA3 |
| Gene ID (NCBI) | 80339 |
| RRID | AB_2882619 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NST1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PNPLA3 belongs to a family of proteins that share a domain that was first identified in patatin, a major soluble protein of potato tubers with nonspecific acyl hydrolase activity. The domain differs from classical lipases by employing a catalytic dyad (Ser-Asp), rather than a catalytic triad, to effect hydrolysis. PNPLA3 is expressed primarily in liver and adipose tissue, in which it partitions to membranes and lipid droplets. Real-time PCR of cDNA from human tissues indicated that PNPLA3 expression was highest in the liver, followed by skin and adipose tissue. PNPLA3 is expressed at the highest levels in adipose tissue of mice. The PNPLA3 protein was expected at 50-70 kDa, as observed; the additional band at approximately 130-150 kDa is a oligomer bands. (PMID: 20385813, PMID: 24931521, PMID: 23023705)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PNPLA3 antibody 67369-1-Ig | Download protocol |
| IHC protocol for PNPLA3 antibody 67369-1-Ig | Download protocol |
| IF protocol for PNPLA3 antibody 67369-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |