验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, A2780 cells |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85834-4-RR targets PNO1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | PNO1 fusion protein Ag14752 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | partner of NOB1 homolog (S. cerevisiae) |
| 别名 | KHRBP1, Partner of NOB1, RNA binding protein PNO1, RNA-binding protein PNO1 |
| 计算分子量 | 252 aa, 28 kDa |
| 观测分子量 | 28-35 kDa |
| GenBank蛋白编号 | BC008304 |
| 基因名称 | PNO1 |
| Gene ID (NCBI) | 56902 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NRX1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human RNA binding gene partner of NOB1 (PNO1) was firstly isolated from human kidney and encodes 35-kDa protein, named PNO1. PNO1 as a ribosome biogenesis factor that contributes to 40S subunit assembly by binding with its partner NOB1. PNO1 is a highly conserved protein with a K homology (KH) domain at its C-terminal and two putative nuclear localization signals at its N-terminal . PNO1 also had great regulatory function in tumor metabolism. (PMID: 38071381, PMID: 33864661)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PNO1 antibody 85834-4-RR | Download protocol |
| IF protocol for PNO1 antibody 85834-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |