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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 85834-4-RR (same clone as 85834-4-PBS)

    WB analysis using 85834-4-RR (same clone as 85834-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85834-4-RR (PNO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85834-4-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 85834-4-RR (same clone as 85834-4-PBS)

    IF Staining of A549 using 85834-4-RR (same clone as 85834-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using PNO1 antibody (85834-4-RR, Clone: 250134F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85834-4-PBS in a different storage buffer formulation.

PNO1 Recombinant antibody, PBS Only

PNO1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85834-4-PBS

产品说明书

CloneNo. 250134F6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

KHRBP1, Partner of NOB1, RNA binding protein PNO1, RNA-binding protein PNO1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85834-4-PBS targets PNO1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 PNO1 fusion protein Ag14752 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 partner of NOB1 homolog (S. cerevisiae)
别名 KHRBP1, Partner of NOB1, RNA binding protein PNO1, RNA-binding protein PNO1
计算分子量 252 aa, 28 kDa
观测分子量 28-35 kDa
GenBank蛋白编号BC008304
基因名称 PNO1
Gene ID (NCBI) 56902
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NRX1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human RNA binding gene partner of NOB1 (PNO1) was firstly isolated from human kidney and encodes 35-kDa protein, named PNO1. PNO1 as a ribosome biogenesis factor that contributes to 40S subunit assembly by binding with its partner NOB1. PNO1 is a highly conserved protein with a K homology (KH) domain at its C-terminal and two putative nuclear localization signals at its N-terminal . PNO1 also had great regulatory function in tumor metabolism. (PMID: 38071381, PMID: 33864661)