验证数据展示
经过测试的应用
| Positive WB detected in | mouse pancreas tissue, rat pancreas tissue |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
26218-1-AP targets PNLIPRP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag23967 Product name: Recombinant human PNLIPRP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 425-469 aa of BC005989 Sequence: RGINLSEPKLGASQITVQSGEDGTEYNFCSSDTVEENVLQSLYPC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pancreatic lipase-related protein 2 |
| 别名 | Cytotoxic T lymphocyte lipase, EC:3.1.1.26, EC:3.1.1.3, Galactolipase, Pancreatic lipase-related protein 2 |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC005989 |
| 基因名称 | PNLIPRP2 |
| Gene ID (NCBI) | 5408 |
| RRID | AB_2880430 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P54317 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Pancreatic lipase-related proteins 1 and 2 (PNLIPRP1 and PNLIPRP2) are highly conserved paralogs within the classic neutral lipase family. Despite structural similarities to canonical pancreatic triglyceride lipase, their catalytic activities diverge drastically. PNLIPRP2 possesses broad-spectrum lipolytic capabilities, digesting galactolipids and phospholipids, which is essential for neonatal milk consumption. Conversely, PNLIPRP1 lacks detectable lipolytic activity against known substrates but functions in human metabolic regulation and exocrine homeostasis.(PMID: 1379598; PMID: 34290745; PMID: 39137110)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PNLIPRP2 antibody 26218-1-AP | Download protocol |
| WB protocol for PNLIPRP2 antibody 26218-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
