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验证数据展示

  • WB analysis using 84894-5-RR (same clone as 84894-5-PBS)

    WB analysis using 84894-5-RR (same clone as 84894-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84894-5-RR (PMS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84894-5-RR (same clone as 84894-5-PBS)

    IHC staining of human tonsillitis using 84894-5-RR (same clone as 84894-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84894-5-RR (PMS2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 84894-5-RR (same clone as 84894-5-PBS)

    FC experiment of MCF-7 using 84894-5-RR (same clone as 84894-5-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PMS2 Recombinant antibody (84894-5-RR, Clone:242455F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84894-5-RR

    Affinity and Kinetic Characterization of 84894-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84894-5-RR against Human PMS2 were performed. The affinity constant is below 1 pM.

PMS2 Recombinant monoclonal antibody, PBS Only

PMS2 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 84894-5-PBS

产品说明书

CloneNo. 242455F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

242455F8, EC:3.1.-.-, HNPCC4, PMS1 protein homolog 2, PMS2CL

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84894-5-PBS targets PMS2 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33508

Product name: Recombinant human PMS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 700-862 aa of BC093921

Sequence: QHATDEKYNFEMLQQHTVLQGQRLIAPQTLNLTAVNEAVLIENLEIFRKNGFDFVIDENAPVTERAKLISLPTSKNWTFGPQDVDELIFMLSDSPGVMCRPSRVKQMFASRACRKSVMIGTALNTSEMKKLITHMGEMDHPWNCPHGRPTMRHIANLGVISQN*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
别名 242455F8, EC:3.1.-.-, HNPCC4, PMS1 protein homolog 2, PMS2CL
计算分子量 862 aa, 96 kDa
观测分子量117 kDa
GenBank蛋白编号BC093921
基因名称 PMS2
Gene ID (NCBI) 5395
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP54278
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS).

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