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PMS1 Monoclonal antibody

PMS1 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A6G3

Cat No : 68413-1-Ig

Print datasheet

Synonyms

DKFZp781M0253, FLJ98259, HNPCC3, hPMS1, PMS1, PMS1 protein homolog 1, PMSL1

验证数据展示

  • WB analysis using 68413-1-Ig

    WB analysis using 68413-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68413-1-Ig (PMS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of Caco-2 using 68413-1-Ig

    IF Staining of Caco-2 using 68413-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using PMS1 antibody (68413-1-Ig, Clone: 1A6G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inCaco-2 cells, HeLa cells, HepG2 cells, LNCaP cells, HEK-293 cells, MOLT-4 cells, K-562 cells, HL-60 cells, Jurkat cells
Positive IF detected inCaco-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68413-1-Ig targets PMS1 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human
Immunogen PMS1 fusion protein Ag28127 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name PMS1 postmeiotic segregation increased 1 (S. cerevisiae)
Synonyms DKFZp781M0253, FLJ98259, HNPCC3, hPMS1, PMS1, PMS1 protein homolog 1, PMSL1
Observed Molecular Weight 106 kDa
GenBank Accession NumberBC096331
Gene Symbol PMS1
Gene ID (NCBI) 5378
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54277
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PMS1 Homolog 1 is a member of the DNA mismatch repair enzymes (MMREs) which is to eliminate the mismatch of insertions and deletions as a consequence of DNA polymerase errors at DNA synthesis in eukaryotes. PMS1 and MLH1 could form a heterodimer MutLβ that belongs to MMREs (PMID:25619773). In the MMR system, the MutS complex recognizes the DNA mispairs firstly, and then the Mlh1-Pms1 and other associated proteins such as PCNA, RFC, ExoI were recruited, inducing Exonuclease 1 (Exo1)-dependent and -independent MMR pathways (PMID:24981171). Mutations in this gene cause hereditary nonpolyposis colorectal cancer(HNPCC), which is also known as Lynch syndrome. It was reported that PMS1 is a widely expressed protein and located in the nucleus. Alternative splicing allows for 4 isoforms of PMS1 protein.

实验方案

Product Specific Protocols
WB protocol for PMS1 antibody 68413-1-IgDownload protocol
IF protocol for PMS1 antibody 68413-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols