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PMS1 Polyclonal antibody

PMS1 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28186-1-AP

Print datasheet

Synonyms

DKFZp781M0253, FLJ98259, HNPCC3, hPMS1, PMS1, PMS1 protein homolog 1, PMSL1

验证数据展示

  • WB analysis using 28186-1-AP

    WB analysis using 28186-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28186-1-AP (PMS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of Caco-2 using 28186-1-AP

    IF Staining of Caco-2 using 28186-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PMS1 antibody (28186-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, MOLT-4 cells, K-562 cells
Positive IF detected inCaco-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28186-1-AP targets PMS1 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human
Immunogen PMS1 fusion protein Ag28132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PMS1 postmeiotic segregation increased 1 (S. cerevisiae)
Synonyms DKFZp781M0253, FLJ98259, HNPCC3, hPMS1, PMS1, PMS1 protein homolog 1, PMSL1
Observed Molecular Weight 106 kDa
GenBank Accession NumberBC096331
Gene Symbol PMS1
Gene ID (NCBI) 5378
RRIDAB_2881083
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54277
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PMS1 Homolog 1 is a member of the DNA mismatch repair enzymes (MMREs) which is to eliminate the mismatch of insertions and deletions as a consequence of DNA polymerase errors at DNA synthesis in eukaryotes. PMS1 and MLH1 could form a heterodimer MutLβ that belongs to MMREs (PMID:25619773). In the MMR system, the MutS complex recognizes the DNA mispairs firstly, and then the Mlh1-Pms1 and other associated proteins such as PCNA, RFC, ExoI were recruited, inducing Exonuclease 1 (Exo1)-dependent and -independent MMR pathways (PMID:24981171). Mutations in this gene cause hereditary nonpolyposis colorectal cancer(HNPCC), which is also known as Lynch syndrome. It was reported that PMS1 is a widely expressed protein and located in the nucleus. Alternative splicing allows for 4 isoforms of PMS1 protein.

实验方案

Product Specific Protocols
WB protocol for PMS1 antibody 28186-1-APDownload protocol
IF protocol for PMS1 antibody 28186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols