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验证数据展示

  • WB analysis of multi-tissue using 10666-1-AP

    WB analysis of multi-tissue using 10666-1-AP

    WB results of 10666-1-AP (PMM2 antibody) on several lysates.

  • WB analysis using 10666-1-AP

    WB analysis using 10666-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10666-1-AP (PMM2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 10666-1-AP

    IP experiment of mouse lung using 10666-1-AP

    IP result of anti-PMM2 (IP:10666-1-AP, 4ug; Detection:10666-1-AP 1:1000) with mouse lung tissue lysate 3600ug.

  • IHC staining of human breast cancer using 10666-1-AP

    IHC staining of human breast cancer using 10666-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10666-1-AP (PMM2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PMM2 antibody included
  • IHC staining of human breast cancer using 10666-1-AP

    IHC staining of human breast cancer using 10666-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10666-1-AP (PMM2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PMM2 antibody included
  • WB analysis using 10666-1-AP

    WB analysis using 10666-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10666-1-AP (PMM2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 10666-1-AP

    IHC staining of human placenta using 10666-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10666-1-AP (PMM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PMM2 antibody included
  • IHC staining of human stomach cancer using 10666-1-AP

    IHC staining of human stomach cancer using 10666-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10666-1-AP (PMM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PMM2 antibody included
  • IHC staining of mouse stomach using 10666-1-AP

    IHC staining of mouse stomach using 10666-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10666-1-AP (PMM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PMM2 antibody included
  • IHC staining of rat stomach using 10666-1-AP

    IHC staining of rat stomach using 10666-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10666-1-AP (PMM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PMM2 antibody included
  • IF Staining of HepG2 using 10666-1-AP

    IF Staining of HepG2 using 10666-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PMM2 antibody (10666-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

PMM2 Polyclonal antibody

PMM2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10666-1-AP

产品说明书

引用文献(17)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

EC:5.4.2.8, phosphomannomutase 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse liver tissue, multi-tissue, HepG2 cellls, mouse testis tissue, rat kidney tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman breast cancer tissue, human placenta tissue, human stomach cancer tissue, mouse stomach tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy® PMM2 Ready-To-Use IHC Kit. PMM2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10666-1-AP targets PMM2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag1031

Product name: Recombinant human PMM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-246 aa of BC008310

Sequence: MAAPGPALCLFDVDGTLTAPRQKITKEMDDFLQKLRQKIKIGVVGGSDFEKVQEQLGNDVVEKYDYVFPENGLVAYKDGKLLCRQNIQSHLGEALIQDLINYCLSYIAKIKLPKKRGTFIEFRNGMLNVSPIGRSCSQEERIEFYELDKKENIRQKFVADLRKEFAGKGLTFSIGGQISFDVFPDGWDKRYCLRHVENDGYKTIYFFGDKTMPGGNDHEIFTDPRTMGYSVTAPEDTRRICELLFS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphomannomutase 2
别名 EC:5.4.2.8, phosphomannomutase 2
计算分子量 28 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC008310
基因名称 PMM2
Gene ID (NCBI) 5373
RRIDAB_2166973
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15305
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PMM2(Phosphomannomutase 2) belongs to the eukaryotic PMM family. It is involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. PMM2 catalyzes the second step in the conversion of fructose-6P to GDP-mannose.

实验方案

Product Specific Protocols
IF protocol for PMM2 antibody 10666-1-APDownload protocol
IHC protocol for PMM2 antibody 10666-1-APDownload protocol
IP protocol for PMM2 antibody 10666-1-APDownload protocol
WB protocol for PMM2 antibody 10666-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Thromb Haemost

N-Glycosylation Deficiency Reduces the Activation of Protein C and Disrupts Endothelial Barrier Integrity

Authors - Tiffany Pascreau
  • KD Validated
View Article
mouseWB

J Proteome Res

Changes in the striatal proteome of YAC128Q mice exhibit gene-environment interactions between mutant huntingtin and manganese.

Authors - Wegrzynowicz Michal M
View Article
humanWB

Mol Genet Metab

Complex metabolic disharmony in PMM2-CDG paves the way to new therapeutic approaches

Authors - Nastassja Himmelreich
View Article
humanWB

J Inherit Metab Dis

Lack of phosphomannomutase 2 affects Xenopus laevis morphogenesis and the non-canonical Wnt5a/Ror2 signalling.

Authors - Nastassja Himmelreich
View Article
humanWB

Dis Model Mech

Repurposing the aldose reductase inhibitor and diabetic neuropathy drug epalrestat for the congenital disorder of glycosylation PMM2-CDG.

Authors - Sangeetha Iyer
View Article
WB

PLoS One

Heterodimerization of Two Pathological Mutants Enhances the Activity of Human Phosphomannomutase2.

Authors - Giuseppina Andreotti
View Article
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