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验证数据展示

  • WB analysis using 28423-1-AP (same clone as 28423-1-PBS)

    WB analysis using 28423-1-AP (same clone as 28423-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28423-1-AP (PMCA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28423-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 28423-1-AP (same clone as 28423-1-PBS)

    IHC staining of mouse brain using 28423-1-AP (same clone as 28423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28423-1-AP (PMCA2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28423-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 28423-1-AP (same clone as 28423-1-PBS)

    IHC staining of rat brain using 28423-1-AP (same clone as 28423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28423-1-AP (PMCA2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28423-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 28423-1-AP (same clone as 28423-1-PBS)

    IF Staining of rat brain using 28423-1-AP (same clone as 28423-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PMCA2 antibody (28423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28423-1-PBS in a different storage buffer formulation.

PMCA2 Polyclonal antibody, PBS Only

PMCA2 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 28423-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28423-1-PBS targets PMCA2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28994

Product name: Recombinant human PMCA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 578-782 aa of NM_001001331

Sequence: LDLKQDYEPVRSQMPEEKLYKVYTFNSVRKSMSTVIKLPDESFRMYSKGASEIVLKKCCKILNGAGEPRVFRPRDRDEMVKKVIEPMACDGLRTICVAYRDFPSSPEPDWDNENDILNELTCICVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAIKCGIIHPGEDFLCLEGKEFNRRIRNEKGEIEQERIDKIW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase, Ca++ transporting, plasma membrane 2
别名
计算分子量 137 kDa
观测分子量 137 kDa
GenBank蛋白编号NM_001001331
基因名称 PMCA2
Gene ID (NCBI) 491
RRIDAB_3086051
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01814
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP2B2, also named as PMCA2, belongs to the cation transport ATPase (P-type) family and type IIB subfamily. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

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