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验证数据展示

  • WB analysis using 28423-1-AP

    WB analysis using 28423-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28423-1-AP (PMCA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28423-1-AP

    IHC staining of mouse brain using 28423-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28423-1-AP (PMCA2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 28423-1-AP

    IHC staining of rat brain using 28423-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28423-1-AP (PMCA2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 28423-1-AP

    IF Staining of rat brain using 28423-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PMCA2 antibody (28423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

PMCA2 Polyclonal antibody

PMCA2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 28423-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

ATP2B2, PMCA2, PMCA2a, PMCA2i

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inunboiled mouse brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28423-1-AP targets PMCA2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PMCA2 fusion protein Ag28994 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase, Ca++ transporting, plasma membrane 2
别名 ATP2B2, PMCA2, PMCA2a, PMCA2i
计算分子量 137 kDa
观测分子量 137 kDa
GenBank蛋白编号NM_001001331
基因名称 PMCA2
Gene ID (NCBI) 491
RRIDAB_3086051
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01814
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP2B2, also named as PMCA2, belongs to the cation transport ATPase (P-type) family and type IIB subfamily. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

实验方案

Product Specific Protocols
WB protocol for PMCA2 antibody 28423-1-APDownload protocol
IHC protocol for PMCA2 antibody 28423-1-APDownload protocol
IF protocol for PMCA2 antibody 28423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols