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验证数据展示

  • WB analysis using 84592-1-RR (same clone as 84592-1-PBS)

    WB analysis using 84592-1-RR (same clone as 84592-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84592-1-RR (PLTP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84592-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 84592-1-RR (same clone as 84592-1-PBS)

    IHC staining of mouse brain using 84592-1-RR (same clone as 84592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84592-1-RR (PLTP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84592-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 84592-1-RR (same clone as 84592-1-PBS)

    IHC staining of mouse brain using 84592-1-RR (same clone as 84592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84592-1-RR (PLTP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84592-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 84592-1-RR (same clone as 84592-1-PBS)

    IF Staining of mouse brain using 84592-1-RR (same clone as 84592-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PLTP antibody (84592-1-RR, Clone: 241583F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84592-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84592-1-RR

    Affinity and Kinetic Characterization of 84592-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84592-1-RR against Human PLTP were performed. The affinity constant is below 1 pM.

PLTP Recombinant monoclonal antibody, PBS Only

PLTP Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84592-1-PBS

产品说明书

CloneNo. 241583F3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

241583F3, Lipid transfer protein II, Phospholipid transfer protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84592-1-PBS targets PLTP in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8171

Product name: Recombinant human PLTP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 163-441 aa of BC005045

Sequence: IDYSLMKDPVASTSNLDMDFRGAFFPLTERNWSLPNRAVEPQLQEEERMVYVAFSEFFFDSAMESYFRAGALQLLLVGDKVPHDLDMLLRATYFGSIVLLSPAVIDSPLKLELRVLAPPRCTIKPSGTTISVTASVTIALVPPDQPEVQLSSMTMDARLSAKMALRGKALRTQLDLRRFRIYSNHSALESLALIPLQAPLKTMLQIGVMPMLNERTWRGVQIPLPEGINFVHEVVTNHAGFLTIGADLHFAKGLREVIEKNRPADVRASTAPTPSTAAV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phospholipid transfer protein
别名 241583F3, Lipid transfer protein II, Phospholipid transfer protein
计算分子量 493 aa, 55 kDa
观测分子量55 kDa
GenBank蛋白编号BC005045
基因名称 PLTP
Gene ID (NCBI) 5360
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP55058
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLTP (phospholipid transfer protein) belongs to the family of lipid-binding and lipid-transfer proteins. PLTP is involved in the distribution and metabolism of high-density lipoprotein ( HDL ) and reverse cholesterol transfer ( RCT ). In plasma, PLTP catalyzes the transfer of phospholipids ( mainly phosphatidylcholine ) between lipoproteins (PMID: 29558025). PLTP has a predicted protein molecular mass of approximately 55 kDa, and molecular weights of 70-80 kDa can also be detected in some literature (PMID: 26160914; 36312255; 29680823; 15342679; 7654777).

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