验证数据展示

WB analysis using 11582-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11582-1-AP
WB result of PLSCR1 antibody (11582-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLSCR1 transfected HEK-293 cells.
IHC staining of mouse spleen using 11582-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11582-1-AP (PLSCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PLSCR1 antibody included
IF Staining of HeLa using 11582-1-AP
Immunofluorescent analysis of HeLa cells using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
WB analysis using 11582-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 11582-1-AP
IP result of anti-PLSCR1 (IP:11582-1-AP, 4ug; Detection:11582-1-AP 1:500) with mouse lung tissue lysate 2800ug.
IF Staining of mouse brain using 11582-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using PLSCR1 antibody (11582-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of rat brain using 11582-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using PLSCR1 antibody (11582-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO验证

PLSCR1 Polyclonal antibody

PLSCR1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, IP, ELISA

Cat No. 11582-1-AP

产品说明书

引用文献(18)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-Fro, IP, ELISA and More (1)

别名

Ca(2+)-dependent phospholipid scramblase 1, EC:3.1.-.-, Mg(2+)-dependent nuclease, MMTRA1B, phospholipid scramblase 1

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (18)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HEK-293 cells, human skeletal muscle tissue, mouse skeletal muscle tissue, mouse lung tissue, A549 cells, HepG2 cells, human placenta tissue
Positive IP detected in	mouse lung tissue
Positive IHC detected in	mouse spleen tissue, human kidney tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-Fro detected in	rat brain tissue, mouse brain tissue
Positive IF/ICC detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy PLSCR1 Ready-To-Use IHC Kit. PLSCR1 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 8 publications below
WB	See 14 publications below
IHC	See 5 publications below
IF	See 8 publications below
IP	See 2 publications below

产品信息

11582-1-AP targets PLSCR1 in WB, IHC, IF/ICC, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IF-Fro, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse
免疫原	CatNo: Ag2172 Product name: Recombinant human PLSCR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-318 aa of BC021100 Sequence: MDKQNSQMNASHPETNLPVGYPPQYPPTAFQGPPGYSGYPGPQVSYPPPPAGHSGPGPAGFPVPNQPVYNQPVYNQPVGAAGVPWMPAPQPPLNCPPGLEYLSQIDQILIHQQIELLEVLTGFETNNKYEIKNSFGQRVYFAAEDTDCCTRNCCGPSRPFTLRIIDNMGQEVITLERPLRCSSCCCPCCLQEIEIQAPPGVPIGYVIQTWHPCLPKFTIQNEKREDVLKISGPCVVCSCCGDVDFEIKSLDEQCVVGKISKHWTGILREAFTDADNFGIQFPLDLDVKMKAVMIGACFLIDFMFFESTGSQEQKSGVW 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	phospholipid scramblase 1
别名	Ca(2+)-dependent phospholipid scramblase 1, EC:3.1.-.-, Mg(2+)-dependent nuclease, MMTRA1B, phospholipid scramblase 1
计算分子量	318 aa, 35 kDa
观测分子量	35 kDa
GenBank蛋白编号	BC021100
基因名称	PLSCR1
Gene ID (NCBI)	5359
RRID	AB_2165659
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	O15162
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

PLSCR1 (Phospholipid scramblase 1) is a member of the family of membrane proteins that mediate the transbilayer movement of phospholipids ("scrambling") in a Ca2+ dependent manner, which was identified as an a type II transmembrane protein involved in the calcium-dependent, nonspecific, rapid redistribution of phospholipids. A cysteine-rich palmitoylation motif [184CCCPCC189] of PLSCR1, a thioester linkage to the sulfhydryl groups of cysteines, controls the transportation of PLSCR1 to the cell membrane or nucleus (PMID: 32292520). PLSCR1 is strongly expressed in response to interferon (IFN) treatment and viral infection (PMID: 35138119, 23487022).

实验方案

Product Specific Protocols
IF protocol for PLSCR1 antibody 11582-1-AP	Download protocol
IHC protocol for PLSCR1 antibody 11582-1-AP	Download protocol
IP protocol for PLSCR1 antibody 11582-1-AP	Download protocol
WB protocol for PLSCR1 antibody 11582-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB,IF	J Clin Invest Phospholipid scramblase-1 regulates innate type 2 inflammation in mouse lung via CRTH2-dependent mechanisms Authors - Ashley Hernandez-Gutierrez View Article
human	WB	PLoS Pathog Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication. Authors - Weiyu Luo View Article
mouse	WB	J Neurosci Phospholipid Scramblase 1 Controls Efficient Neurotransmission and Synaptic Vesicle Retrieval at Cerebellar Synapses Authors - Margherita Caputo KD Validated View Article
human	WB,IF	J Proteome Res An Interactome Map Reveals Phospholipid Scramblase 1 as a Novel Regulator of Hepatitis B Virus X Protein. Authors - Yanzhi Yuan KD Validated View Article
human	WB,IP,IF	Cancer Sci Nuclear accumulation of KPNA2 impacts radioresistance through positive regulation of the PLSCR1-STAT1 loop in lung adenocarcinoma. Authors - Wei-Chao Liao KD Validated View Article
mouse	WB	J Leukoc Biol Phospholipid scramblase 1 is required for β2-glycoprotein I binding in hypoxia and reoxygenation-induced endothelial inflammation. Authors - Emily Archer Slone View Article

验证数据展示

WB analysis using 11582-1-AP

WB analysis of HEK-293 using 11582-1-AP

IHC staining of mouse spleen using 11582-1-AP

IF Staining of HeLa using 11582-1-AP

WB analysis using 11582-1-AP

IP experiment of mouse lung using 11582-1-AP

IF Staining of mouse brain using 11582-1-AP

IF Staining of rat brain using 11582-1-AP

PLSCR1 Polyclonal antibody

PLSCR1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Phospholipid scramblase-1 regulates innate type 2 inflammation in mouse lung via CRTH2-dependent mechanisms

Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication.

Phospholipid Scramblase 1 Controls Efficient Neurotransmission and Synaptic Vesicle Retrieval at Cerebellar Synapses

An Interactome Map Reveals Phospholipid Scramblase 1 as a Novel Regulator of Hepatitis B Virus X Protein.

Nuclear accumulation of KPNA2 impacts radioresistance through positive regulation of the PLSCR1-STAT1 loop in lung adenocarcinoma.

Phospholipid scramblase 1 is required for β2-glycoprotein I binding in hypoxia and reoxygenation-induced endothelial inflammation.