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验证数据展示

  • WB analysis using 86323-1-RR (same clone as 86323-1-PBS)

    WB analysis using 86323-1-RR (same clone as 86323-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86323-1-RR (PLS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86323-1-PBS in a different storage buffer formulation.

  • WB analysis using 86323-1-RR (same clone as 86323-1-PBS)

    WB analysis using 86323-1-RR (same clone as 86323-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86323-1-RR (PLS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86323-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 86323-1-RR (same clone as 86323-1-PBS)

    IHC staining of human skin cancer using 86323-1-RR (same clone as 86323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86323-1-RR (PLS3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86323-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 86323-1-RR (same clone as 86323-1-PBS)

    IHC staining of human skin cancer using 86323-1-RR (same clone as 86323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86323-1-RR (PLS3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86323-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86323-1-RR (same clone as 86323-1-PBS)

    IF Staining of HeLa using 86323-1-RR (same clone as 86323-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLS3 antibody (86323-1-RR, Clone: 250908B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86323-1-PBS in a different storage buffer formulation.

PLS3 Recombinant monoclonal antibody, PBS Only

PLS3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86323-1-PBS

产品说明书

CloneNo. 250908B6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Plastin 3, Plastin-3, T plastin, T-fimbrin, T-plastin

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86323-1-PBS targets PLS3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 plastin 3 (T isoform)
别名 Plastin 3, Plastin-3, T plastin, T-fimbrin, T-plastin
计算分子量 71 kDa
观测分子量 68 kDa
GenBank蛋白编号NM_005032
基因名称 PLS3
Gene ID (NCBI) 5358
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP13797
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLS3, also named T-plastin and Plastin-3, is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It plays an important role in leukocyte function. L-plastin plays an important role in leukocyte function, and T-plastin is possibly involved in DNA repair. Both T- and L-plastin are implicated in several diseases, and L-plastin is considered to be a valuable marker for cancer (PMID: 15960882).

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