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PLS3 Polyclonal antibody

PLS3 Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12917-1-AP

Print datasheet

Synonyms

Plastin 3, plastin 3 (T isoform), PLS3, T plastin

验证数据展示

  • WB analysis using 12917-1-AP

    WB analysis using 12917-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12917-1-AP (PLS3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 12917-1-AP

    WB analysis of A431 using 12917-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12917-1-AP (PLS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of A431 using 12917-1-AP

    IP experiment of A431 using 12917-1-AP

    IP result of anti-PLS3 (IP:12917-1-AP, 4ug; Detection:12917-1-AP 1:500) with A431 cells lysate 2640 ug.

  • IF Staining of A431 using 12917-1-AP

    IF Staining of A431 using 12917-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using PLS3 antibody (12917-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells
Positive IP detected inA431 cells
Positive IF detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12917-1-AP targets PLS3 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PLS3 fusion protein Ag3574 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name plastin 3 (T isoform)
Synonyms Plastin 3, plastin 3 (T isoform), PLS3, T plastin
Calculated Molecular Weight 630 aa, 71 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC039049
Gene Symbol PLS3
Gene ID (NCBI) 5358
RRIDAB_2877892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13797
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLS3, also named T-plastin and Plastin-3, is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It plays an important role in leukocyte function. L-plastin plays an important role in leukocyte function, and T-plastin is possibly involved in DNA repair. Both T- and L-plastin are implicated in several diseases, and L-plastin is considered to be a valuable marker for cancer (PMID: 15960882).

实验方案

Product Specific Protocols
WB protocol for PLS3 antibody 12917-1-APDownload protocol
IF protocol for PLS3 antibody 12917-1-APDownload protocol
IP protocol for PLS3 antibody 12917-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development.

Authors - Jasmin Morandell
View Article