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验证数据展示

  • IHC staining of human brain using 11914-1-AP (same clone as 11914-1-PBS)

    IHC staining of human brain using 11914-1-AP (same clone as 11914-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11914-1-AP (PLRG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 11914-1-AP (same clone as 11914-1-PBS)

    IHC staining of human brain using 11914-1-AP (same clone as 11914-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11914-1-AP (PLRG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 11914-1-AP (same clone as 11914-1-PBS)

    WB analysis of K-562 using 11914-1-AP (same clone as 11914-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 11914-1-AP (PLRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 11914-1-AP (same clone as 11914-1-PBS)

    IP experiment of HEK-293 using 11914-1-AP (same clone as 11914-1-PBS)

    IP result of anti-PLRG1 (IP:11914-1-AP, 4ug; Detection:11914-1-AP 1:1000) with HEK-293 cells lysate 1400ug. This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 11914-1-AP (same clone as 11914-1-PBS)

    WB analysis of HEK-293 using 11914-1-AP (same clone as 11914-1-PBS)

    WB result of PLRG1 antibody (11914-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLRG1 transfected HEK-293 cells. This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PLRG1 Polyclonal antibody, PBS Only

PLRG1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11914-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11914-1-PBS targets PLRG1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2527

Product name: Recombinant human PLRG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 203-505 aa of BC020786

Sequence: VEPGNQWFVTGSADRTIKIWDLASGKLKLSLTGHISTVRGVIVSTRSPYLFSCGEDKQVKCWDLEYNKVIRHYHGHLSAVYGLDLHPTIDVLVTCSRDSTARIWDVRTKASVHTLSGHTNAVATVRCQAAEPQIITGSHDTTIRLWDLVAGKTRVTLTNHKKSVRAVVLHPRHYTFASGSPDNIKQWKFPDGSFIQNLSGHNAIINTLTVNSDGVLVSGADNGTMHLWDWRTGYNFQRVHAAVQPGSLDSESGIFACAFDQSESRLLTAEADKTIKVYREDDTATEETHPVSWKPEIIKRKRF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pleiotropic regulator 1 (PRL1 homolog, Arabidopsis)
别名
计算分子量 514 aa, 57 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC020786
基因名称 PLRG1
Gene ID (NCBI) 5356
RRIDAB_2165608
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43660
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLRG1, identifed as a component of the CDC5L complex, is one spliceosomal protein highly conserved across species. It has been shown to be essential for pre-mRNA splicing in yeast and humans.

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