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验证数据展示

  • IHC staining of human brain using 11914-1-AP

    IHC staining of human brain using 11914-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11914-1-AP (PLRG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 11914-1-AP

    IHC staining of human brain using 11914-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11914-1-AP (PLRG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of K-562 using 11914-1-AP

    WB analysis of K-562 using 11914-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11914-1-AP (PLRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 11914-1-AP

    IP experiment of HEK-293 using 11914-1-AP

    IP result of anti-PLRG1 (IP:11914-1-AP, 4ug; Detection:11914-1-AP 1:1000) with HEK-293 cells lysate 1400ug.

  • WB analysis of HEK-293 using 11914-1-AP

    WB analysis of HEK-293 using 11914-1-AP

    WB result of PLRG1 antibody (11914-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLRG1 transfected HEK-293 cells.

  • Featured Product
  • KD/KO验证

PLRG1 Polyclonal antibody

PLRG1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11914-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA

别名

Pleiotropic regulator 1, PLRG1, PRL1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, HEK-293 cells, BxPC-3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

11914-1-AP targets PLRG1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag2527

Product name: Recombinant human PLRG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 203-505 aa of BC020786

Sequence: VEPGNQWFVTGSADRTIKIWDLASGKLKLSLTGHISTVRGVIVSTRSPYLFSCGEDKQVKCWDLEYNKVIRHYHGHLSAVYGLDLHPTIDVLVTCSRDSTARIWDVRTKASVHTLSGHTNAVATVRCQAAEPQIITGSHDTTIRLWDLVAGKTRVTLTNHKKSVRAVVLHPRHYTFASGSPDNIKQWKFPDGSFIQNLSGHNAIINTLTVNSDGVLVSGADNGTMHLWDWRTGYNFQRVHAAVQPGSLDSESGIFACAFDQSESRLLTAEADKTIKVYREDDTATEETHPVSWKPEIIKRKRF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pleiotropic regulator 1 (PRL1 homolog, Arabidopsis)
别名 Pleiotropic regulator 1, PLRG1, PRL1
计算分子量 514 aa, 57 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC020786
基因名称 PLRG1
Gene ID (NCBI) 5356
RRIDAB_2165608
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43660
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLRG1, identifed as a component of the CDC5L complex, is one spliceosomal protein highly conserved across species. It has been shown to be essential for pre-mRNA splicing in yeast and humans.

实验方案

Product Specific Protocols
WB protocol for PLRG1 antibody 11914-1-APDownload protocol
IHC protocol for PLRG1 antibody 11914-1-APDownload protocol
IP protocol for PLRG1 antibody 11914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.

Authors - Shuai Hou
View Article
humanWB

Cell Death Differ

USP42 drives nuclear speckle mRNA splicing via directing dynamic phase separation to promote tumorigenesis.

Authors - Shuyan Liu
View Article