• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PLOD3 Polyclonal antibody

PLOD3 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat No : CL594-11027

Print datasheet

Synonyms

LH3, Lysyl hydroxylase 3, PLOD3

验证数据展示

  • IF Staining of HepG2 using CL594-11027

    IF Staining of HepG2 using CL594-11027

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 PLOD3 antibody (CL594-11027) at dilution of 1:200.

经过测试的应用

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 11027-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-11027 targets PLOD3 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen PLOD3 fusion protein Ag1480 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Synonyms LH3, Lysyl hydroxylase 3, PLOD3
Calculated Molecular Weight 738 aa, 85 kDa
Observed Molecular Weight 80-85 kDa
GenBank Accession NumberBC011674
Gene Symbol PLOD3
Gene ID (NCBI) 8985
RRIDAB_2919757
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60568
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.

实验方案

Product Specific Protocols
IF protocol for CL594 PLOD3 antibody CL594-11027Download protocol
Standard Protocols
Click here to view our Standard Protocols