验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 11027-1-AP
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-11027 targets PLOD3 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | PLOD3 fusion protein Ag1480 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 |
别名 | LH3, Lysyl hydroxylase 3, PLOD3 |
计算分子量 | 738 aa, 85 kDa |
观测分子量 | 80-85 kDa |
GenBank蛋白编号 | BC011674 |
基因名称 | PLOD3 |
Gene ID (NCBI) | 8985 |
RRID | AB_2919757 |
偶联类型 | CoraLite®594 Fluorescent Dye |
最大激发/发射波长 | 588 nm / 604 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O60568 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 PLOD3 antibody CL594-11027 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |