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验证数据展示

  • WB analysis using 88222-2-RR

    WB analysis using 88222-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88222-2-RR (PLOD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 88222-2-RR

    WB analysis using 88222-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88222-2-RR (PLOD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 88222-2-RR

    WB analysis of NIH/3T3 using 88222-2-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 88222-2-RR (PLOD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

PLOD2 Recombinant monoclonal antibody

PLOD2 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 88222-2-RR

产品说明书

CloneNo. 260421A7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

EC:1.14.11.4, Lysyl hydroxylase 2, PLOD2/LH2, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDU 145 cells, HSC-T6 cells, NIH/3T3 cells, A549 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PLOD2 Ready-To-Use IHC Kit. PLOD2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

88222-2-RR targets PLOD2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15614

Product name: Recombinant human PLOD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 459-540 aa of BC037169

Sequence: PYMANVYLIKGKTLRSEMNERNYFVRDKLDPDMALCRNAREMTLQREKDSPTPETFQMLSPPKGVFMYISNRHEFGRLLSTA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
别名 EC:1.14.11.4, Lysyl hydroxylase 2, PLOD2/LH2, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2
计算分子量 758 aa, 85 kDa
观测分子量 85 kDa
GenBank蛋白编号BC037169
基因名称 PLOD2
Gene ID (NCBI) 5352
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00469
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.

实验方案

Product Specific Protocols
WB protocol for PLOD2 antibody 88222-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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