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验证数据展示

  • WB analysis using 88222-2-RR (same clone as 88222-2-PBS)

    WB analysis using 88222-2-RR (same clone as 88222-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88222-2-RR (PLOD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88222-2-PBS in a different storage buffer formulation.

  • WB analysis using 88222-2-RR (same clone as 88222-2-PBS)

    WB analysis using 88222-2-RR (same clone as 88222-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88222-2-RR (PLOD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88222-2-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 88222-2-RR (same clone as 88222-2-PBS)

    WB analysis of NIH/3T3 using 88222-2-RR (same clone as 88222-2-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 88222-2-RR (PLOD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88222-2-PBS in a different storage buffer formulation.

PLOD2 Recombinant monoclonal antibody, PBS Only

PLOD2 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 88222-2-PBS

产品说明书

CloneNo. 260421A7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

EC:1.14.11.4, Lysyl hydroxylase 2, PLOD2/LH2, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PLOD2 Ready-To-Use IHC Kit. PLOD2 primary antibody included.

产品信息

88222-2-PBS targets PLOD2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15614

Product name: Recombinant human PLOD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 459-540 aa of BC037169

Sequence: PYMANVYLIKGKTLRSEMNERNYFVRDKLDPDMALCRNAREMTLQREKDSPTPETFQMLSPPKGVFMYISNRHEFGRLLSTA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
别名 EC:1.14.11.4, Lysyl hydroxylase 2, PLOD2/LH2, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2
计算分子量 758 aa, 85 kDa
观测分子量 85 kDa
GenBank蛋白编号BC037169
基因名称 PLOD2
Gene ID (NCBI) 5352
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00469
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.

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