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- KD/KO Validated
PLDN Polyclonal antibody
PLDN Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, K-562 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10891-2-AP targets PLDN in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | PLDN fusion protein Ag1337 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | pallidin homolog (mouse) |
Synonyms | BLOC1S6, PA, PALLID, Pallid protein homolog, Pallidin, pallidin homolog (mouse), PLDN |
Calculated Molecular Weight | 23~24 kDa |
Observed Molecular Weight | 25 kDa |
GenBank Accession Number | BC004819 |
Gene Symbol | PLDN |
Gene ID (NCBI) | 26258 |
RRID | AB_2164174 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9UL45 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PLDN antibody 10891-2-AP | Download protocol |
IHC protocol for PLDN antibody 10891-2-AP | Download protocol |
IP protocol for PLDN antibody 10891-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Curr Biol BLOC-1 Brings Together the Actin and Microtubule Cytoskeletons to Generate Recycling Endosomes.
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Am J Respir Cell Mol Biol Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.
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Elife Mechanical stimulation promotes enthesis injury repair by mobilizing Prrx1+ cells via ciliary TGF-β signaling. |