验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells, mouse cerebellum tissue, rat cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33683-1-AP targets PLCB4 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38268 Product name: Recombinant human PLCB4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 900-1110 aa of NM_182797.2 Sequence: ALASGVEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKSTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSEMINTHSAEEQEIRDLHLSQQCELLKKLLINAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVREL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phospholipase C, beta 4 |
| 别名 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4, EC:3.1.4.11, Phosphoinositide phospholipase C-beta-4, Phospholipase C-beta-4, PLC-beta-4 |
| 计算分子量 | 134 kDa，1175aa |
| 观测分子量 | 134 kDa |
| GenBank蛋白编号 | NM_182797.2 |
| 基因名称 | PLCB4 |
| Gene ID (NCBI) | 5332 |
| RRID | AB_3743049 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q15147 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Phospholipase C beta 4 (PLCB4) is a direct effector of the endothelin receptor signaling pathway that plays an essential role in lower jaw and middle ear structures development (PMID: 35284927). Additionally, PLCB4 has an critical role in osteosarcoma progression and may serve as a novel diagnostic or therapeutic target in pediatric patients with acute myeloid leukemia (PMID: 39987366; 31788080).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLCB4 antibody 33683-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
