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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • WB analysis of Sp2/0 using 66668-1-Ig (same clone as 66668-1-PBS)

    WB analysis of Sp2/0 using 66668-1-Ig (same clone as 66668-1-PBS)

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66668-1-Ig (same clone as 66668-1-PBS)

    IHC staining of human breast cancer using 66668-1-Ig (same clone as 66668-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66668-1-Ig (same clone as 66668-1-PBS)

    IHC staining of human breast cancer using 66668-1-Ig (same clone as 66668-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

PLCB3 Monoclonal antibody, PBS Only

PLCB3 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66668-1-PBS

产品说明书

CloneNo. 1B8B3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Phospholipase C-beta-3, Phospholipase C beta 3, Phosphoinositide phospholipase C-beta-3, EC:3.1.4.11, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66668-1-PBS targets PLCB3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15845

Product name: Recombinant human PLCB3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 885-1234 aa of BC142681

Sequence: AQAGQETCQDTQSQQLGSQPSSNPTPSPLDASPRRPPGPTTSPASTSLSSPGQRDDLIASILSEVAPTPLDELRGHKALVKLRSRQERDLRELRKKHQRKAVTLTRRLLDGLAQAQAEGRCRLRPGALGGAADVEDTKEGEDEAKRYQEFQNRQVQSLLELREAQVDAEAQRRLEHLRQALQRLREVVLDANTTQFKRLKEMNEREKKELQKILDRKRHNSISEAKMRDKHKKEAELTEINRRHITESVNSIRRLEEAQKQRHDRLVAGQQQVLQQLAEEEPKLLAQLAQECQEQRARLPQEIRRSLLGEMPEGLGDGPLVACASNGHAPGSSGHLSGADSESQEENTQL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phospholipase C, beta 3 (phosphatidylinositol-specific)
别名 Phospholipase C-beta-3, Phospholipase C beta 3, Phosphoinositide phospholipase C-beta-3, EC:3.1.4.11, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3
计算分子量 1234 aa, 139 kDa
观测分子量 150 kDa
GenBank蛋白编号BC142681
基因名称 PLCB3
Gene ID (NCBI) 5331
RRIDAB_2882022
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ01970
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq/11 and/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.