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验证数据展示

  • WB analysis using 86752-3-RR (same clone as 86752-3-PBS)

    WB analysis using 86752-3-RR (same clone as 86752-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86752-3-RR (ALPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86752-3-PBS in a different storage buffer formulation.

  • WB analysis using 86752-3-RR (same clone as 86752-3-PBS)

    WB analysis using 86752-3-RR (same clone as 86752-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86752-3-RR (ALPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86752-3-PBS in a different storage buffer formulation.

  • Affinity of 86752-3-RR

    Affinity of 86752-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86752-3-RR against Human PLAP were performed. The affinity constant is 2.06 nM.

PLAP Recombinant monoclonal antibody, PBS Only

PLAP Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86752-3-PBS

产品说明书

CloneNo. 251261A10

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, Indirect ELISA

别名

ALPP, EC:3.1.3.1, PALP, PLAP-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ALPP/PLAP Ready-To-Use IHC Kit. ALPP/PLAP primary antibody included.

产品信息

86752-3-PBS targets PLAP in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Eg3065

Product name: Recombinant human ALPP protein (C-rFc)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 23-506 aa of NM_001632.4

Sequence: IIPVEEENPDFWNREAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 alkaline phosphatase, placental (Regan isozyme)
别名 ALPP, EC:3.1.3.1, PALP, PLAP-1
计算分子量58 kDa
观测分子量 66 kDa
GenBank蛋白编号NM_001632.4
基因名称 PLAP
Gene ID (NCBI) 250
RRIDAB_3745102
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05187
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.

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