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验证数据展示

  • WB analysis using 29990-1-AP

    WB analysis using 29990-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29990-1-AP (PLA2G4E antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 29990-1-AP

    IF Staining of A431 using 29990-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLA2G4E antibody (29990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PLA2G4E Polyclonal antibody

PLA2G4E Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29990-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

phospholipase A2, group IVE, PLA2G4E Ens

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, K-562 cells, NIH/3T3 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29990-1-AP targets PLA2G4E in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag32314

Product name: Recombinant human PLA2G4E protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 713-868 aa of BC101584

Sequence: LRPERKADLIIHLNYCAGSQTKPLKQTCEYCTVQNIPFPKYELPDENENLKECYLMENPQEPDAPIVTFFPLINDTFRKYKAPGVERSPEELEQGQVDIYGPKTPYATKELTYTEATFDKLVKLSEYNILNNKDTLLQALRLAVEKKKRLKGQCPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase A2, group IVE
别名 phospholipase A2, group IVE, PLA2G4E Ens
计算分子量 868 aa, 99 kDa
观测分子量98 kDa
GenBank蛋白编号BC101584
基因名称 PLA2G4E
Gene ID (NCBI) 123745
RRIDAB_2935494
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3MJ16
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLA2G4E (Phospholipase A2 group IVE), also known as cPLA2-epsilon. Calcium-dependent N-acyltransferase is involved in the biosynthesis of N-acyl ethanolamines (NAEs) in the brain (PMID:29447909). Transfers the sn-1 fatty acyl chain of phosphatidylcholine (fatty acyl donor) to the amine group of phosphatidylethanolamine (fatty acyl acceptor) to generate N-acyl phosphatidylethanolamine (NAPE). Similarly can use plasmenylethanolamine as a fatty acyl acceptor to form N-acyl plasmenylethanolamine (N-Acyl-PlsEt). Both NAPE and N-Acyl-PlsEt can serve as precursors of bioactive NAEs like N-arachidonoyl phosphatidylethanolamine also called anandamide (PMID:29447909; 30517655).

实验方案

Product Specific Protocols
IF protocol for PLA2G4E antibody 29990-1-APDownload protocol
WB protocol for PLA2G4E antibody 29990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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