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PLA2G4E Polyclonal antibody

PLA2G4E Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29990-1-AP

Print datasheet

Synonyms

FLJ45651, phospholipase A2, group IVE, PLA2G4E, PLA2G4E Ens

验证数据展示

  • WB analysis using 29990-1-AP

    WB analysis using 29990-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29990-1-AP (PLA2G4E antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 29990-1-AP

    IF Staining of A431 using 29990-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLA2G4E antibody (29990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA431 cells, K-562 cells, NIH/3T3 cells
Positive IF detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29990-1-AP targets PLA2G4E in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen PLA2G4E fusion protein Ag32314 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase A2, group IVE
Synonyms FLJ45651, phospholipase A2, group IVE, PLA2G4E, PLA2G4E Ens
Calculated Molecular Weight 868 aa, 99 kDa
Observed Molecular Weight98 kDa
GenBank Accession NumberBC101584
Gene Symbol PLA2G4E
Gene ID (NCBI) 123745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLA2G4E (Phospholipase A2 group IVE), also known as cPLA2-epsilon. Calcium-dependent N-acyltransferase is involved in the biosynthesis of N-acyl ethanolamines (NAEs) in the brain (PMID:29447909). Transfers the sn-1 fatty acyl chain of phosphatidylcholine (fatty acyl donor) to the amine group of phosphatidylethanolamine (fatty acyl acceptor) to generate N-acyl phosphatidylethanolamine (NAPE). Similarly can use plasmenylethanolamine as a fatty acyl acceptor to form N-acyl plasmenylethanolamine (N-Acyl-PlsEt). Both NAPE and N-Acyl-PlsEt can serve as precursors of bioactive NAEs like N-arachidonoyl phosphatidylethanolamine also called anandamide (PMID:29447909; 30517655).

实验方案

Product Specific Protocols
WB protocol for PLA2G4E antibody 29990-1-APDownload protocol
IF protocol for PLA2G4E antibody 29990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols