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验证数据展示

  • WB analysis using 17646-1-AP (same clone as 17646-1-PBS)

    WB analysis using 17646-1-AP (same clone as 17646-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17646-1-AP (PLA2G3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17646-1-PBS in a different storage buffer formulation.

  • IP experiment of human milk using 17646-1-AP (same clone as 17646-1-PBS)

    IP experiment of human milk using 17646-1-AP (same clone as 17646-1-PBS)

    IP result of anti-PLA2G3 (IP:17646-1-AP, 4ug; Detection:17646-1-AP 1:500) with human milk lysate 3600 ug. This data was developed using the same antibody clone with 17646-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 17646-1-AP (same clone as 17646-1-PBS)

    IHC staining of human colon cancer using 17646-1-AP (same clone as 17646-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17646-1-AP (PLA2G3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17646-1-PBS in a different storage buffer formulation.

PLA2G3 Polyclonal antibody, PBS Only

PLA2G3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 17646-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

EC:3.1.1.4, GIII sPLA2, Phosphatidylcholine 2-acylhydrolase 3, phospholipase A2, group III, sPLA2 III

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17646-1-PBS targets PLA2G3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11845

Product name: Recombinant human PLA2G3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 160-509 aa of BC025316

Sequence: GVGDSAGNSSELGVFQGPDLCCREHDRCPQNISPLQYNYGIRNYRFHTISHCDCDTRFQQCLQNQHDSISDIVGVAFFNVLEIPCFVLEEQEACVAWYWWGGCRMYGTVPLARLQPRTFYNASWSSRATSPTPSSRSPAPPKPRQKQHLRKGPPHQKGSKRPSKANTTALQDPMVSPRLDVAPTGLQGPQGGLKPQGARWVCRSFRRHLDQCEHQIGPREIEFQLLNSAQEPLFHCNCTRRLARFLRLHSPPEVTNMLWELLGTTCFKLAPPLDCVEGKNCSRDPRAIRVSARHLRRLQQRRHQLQDKGTDERQPWPSEPLRGPMSFYNQCLQLTQAARRPDRQQKSWSQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase A2, group III
别名 EC:3.1.1.4, GIII sPLA2, Phosphatidylcholine 2-acylhydrolase 3, phospholipase A2, group III, sPLA2 III
计算分子量 509 aa, 57 kDa
观测分子量57 kDa
GenBank蛋白编号BC025316
基因名称 PLA2G3
Gene ID (NCBI) 50487
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZ20
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The PLA2G3 protein (full name: Phospholipase A2 Group III) is a member of the secreted phospholipase A2 (sPLA2) family. It is a calcium-dependent secretory protein whose primary biochemical function is to catalyze the hydrolysis of the sn-2 position of phospholipids, releasing arachidonic acid and lysophospholipids. It is an enzyme involved in the hydrolysis of membrane phospholipids and lipid metabolism, playing important roles in the development and progression of innate immunity, inflammatory diseases (such as asthma and psoriasis), atherosclerosis, and certain cancers (such as colorectal cancer and ovarian cancer).

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