验证数据展示
经过测试的应用
| Positive WB detected in | human milk |
| Positive IP detected in | human milk |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17646-1-AP targets PLA2G3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag11845 Product name: Recombinant human PLA2G3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 160-509 aa of BC025316 Sequence: GVGDSAGNSSELGVFQGPDLCCREHDRCPQNISPLQYNYGIRNYRFHTISHCDCDTRFQQCLQNQHDSISDIVGVAFFNVLEIPCFVLEEQEACVAWYWWGGCRMYGTVPLARLQPRTFYNASWSSRATSPTPSSRSPAPPKPRQKQHLRKGPPHQKGSKRPSKANTTALQDPMVSPRLDVAPTGLQGPQGGLKPQGARWVCRSFRRHLDQCEHQIGPREIEFQLLNSAQEPLFHCNCTRRLARFLRLHSPPEVTNMLWELLGTTCFKLAPPLDCVEGKNCSRDPRAIRVSARHLRRLQQRRHQLQDKGTDERQPWPSEPLRGPMSFYNQCLQLTQAARRPDRQQKSWSQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phospholipase A2, group III |
| 别名 | EC:3.1.1.4, GIII sPLA2, Phosphatidylcholine 2-acylhydrolase 3, phospholipase A2, group III, sPLA2 III |
| 计算分子量 | 509 aa, 57 kDa |
| 观测分子量 | 57 kDa |
| GenBank蛋白编号 | BC025316 |
| 基因名称 | PLA2G3 |
| Gene ID (NCBI) | 50487 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZ20 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The PLA2G3 protein (full name: Phospholipase A2 Group III) is a member of the secreted phospholipase A2 (sPLA2) family. It is a calcium-dependent secretory protein whose primary biochemical function is to catalyze the hydrolysis of the sn-2 position of phospholipids, releasing arachidonic acid and lysophospholipids. It is an enzyme involved in the hydrolysis of membrane phospholipids and lipid metabolism, playing important roles in the development and progression of innate immunity, inflammatory diseases (such as asthma and psoriasis), atherosclerosis, and certain cancers (such as colorectal cancer and ovarian cancer).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PLA2G3 antibody 17646-1-AP | Download protocol |
| IP protocol for PLA2G3 antibody 17646-1-AP | Download protocol |
| WB protocol for PLA2G3 antibody 17646-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
