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验证数据展示

  • WB analysis of HEK-293 using 14608-1-AP

    WB analysis of HEK-293 using 14608-1-AP

    WB result of PKN2 antibody (14608-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKN2 transfected HEK-293 cells.

  • IP experiment of COLO 320 using 14608-1-AP

    IP experiment of COLO 320 using 14608-1-AP

    IP result of anti-PKN2 (IP:14608-1-AP, 4ug; Detection:14608-1-AP 1:1000) with COLO 320 cells lysate 2560 ug.

  • IF Staining of HepG2 using 14608-1-AP

    IF Staining of HepG2 using 14608-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PKN2 antibody (14608-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 14608-1-AP

    FC experiment of HepG2 using 14608-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PKN2 (14608-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • WB analysis using 14608-1-AP

    WB analysis using 14608-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 14608-1-AP

    IHC staining of human stomach using 14608-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14608-1-AP (PKN2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PKN2 antibody included
  • IHC staining of human lung cancer using 14608-1-AP

    IHC staining of human lung cancer using 14608-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14608-1-AP (PKN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PKN2 antibody included
  • IHC staining of human colon cancer using 14608-1-AP

    IHC staining of human colon cancer using 14608-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14608-1-AP (PKN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PKN2 antibody included
  • IHC staining of human colon cancer using 14608-1-AP

    IHC staining of human colon cancer using 14608-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14608-1-AP (PKN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PKN2 antibody included
  • Featured Product
  • KD/KO验证

PKN2 Polyclonal antibody

PKN2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 14608-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

EC:2.7.11.13, Pak 2, PAK2, PKN gamma, PRK2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA2780 cells, K-562 cells, HeLa cells, HEK-293 cells, COLO 320 cells, SKOV-3 cells, HEK-293T cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PKN2 Ready-To-Use IHC Kit. PKN2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14608-1-AP targets PKN2 in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, ChIP
经测试反应性 human
文献引用反应性human, pig
免疫原

CatNo: Ag6164

Product name: Recombinant human PKN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-60 aa of BC062620

Sequence: MDKKVKPPFIPTIRGREDVSNFDDEFTSEAPILTPPREPRILSEEEQEMFRDFDYIADWC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase N2
别名 EC:2.7.11.13, Pak 2, PAK2, PKN gamma, PRK2
计算分子量 112 kDa
观测分子量 130 kDa
GenBank蛋白编号BC062620
基因名称 PKN2
Gene ID (NCBI) 5586
RRIDAB_10638487
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16513
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for PKN2 antibody 14608-1-APDownload protocol
IF protocol for PKN2 antibody 14608-1-APDownload protocol
IHC protocol for PKN2 antibody 14608-1-APDownload protocol
IP protocol for PKN2 antibody 14608-1-APDownload protocol
WB protocol for PKN2 antibody 14608-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Cell Int

Upregulation of SNTB1 correlates with poor prognosis and promotes cell growth by negative regulating PKN2 in colorectal cancer

Authors - Liya Liu
View Article
pigWB,IHC

Reprod Domest Anim

miR-26a inhibits proliferation and promotes apoptosis in porcine immature Sertoli cells by targeting the PAK2 gene.

Authors - Maoliang Ran
View Article
humanChIP

Mol Med

PKN2 enhances the immunosuppressive activity of polymorphonuclear myeloid-derived suppressor cells in esophageal carcinoma by mediating fatty acid oxidation

Authors - Xiao Fu
View Article
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