验证数据展示
经过测试的应用
| Positive WB detected in | U-87 MG cells, U-251 cells, HT-1080 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86366-2-RR targets PJA2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag3434 Product name: Recombinant human PJA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC030826 Sequence: MSQYTEKEPAAMDQESGKAVWPKPAGGYQTITGRRYGRRHAYVSFKPCMTRHERSLGRAGDDYEVLELDDVPKENSSGSSPLDQVDSSLPSEPIFEKSETEIPTCGSALNQTTESSQSFVAVHHSEEGRDTLGSSTNLHNHSEGEYIPGACSASSVQNGIALVHTDSYDPDGKHGEDNDHLQLSAEVVEGSRYQESLGNTVFELENREAEAYTGLSPPVPSFNCEVRDEFEELDSVPLVKSSAGDTEFVHQNSQEIQRSSQDEMVSTKQQNNTSQERQTEHSPEDAACGPGHICSERNTN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | praja ring finger 2 |
| 别名 | E3 ubiquitin-protein ligase Praja-2, EC:2.3.2.27, KIAA0438, Neurodap1, praja ring finger 2 |
| 计算分子量 | 708 aa, 78 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC030826 |
| 基因名称 | PJA2 |
| Gene ID (NCBI) | 9867 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O43164 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PJA2 is responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. It is also essential for PKA-mediated long-term memory processes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PJA2 antibody 86366-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
