验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
34244-1-AP targets PIWIL2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | rat |
| 免疫原 |
CatNo: Ag38831 Product name: Recombinant human PIWIL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC025995 Sequence: MDPFRPSFRGQSPIHPSQCQAVRMPGCWPQASKPLDPALGRGAPAGRGHVFGKPEEPSTQRGPAQRESVGLVSMFRGLGIETVSKTPLKREMLPSGRGILGRGLSANLVRKDREELSPTFWDPKVLAAGDSKMAETSVGWSRTLGRGSSD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | piwi-like 2 (Drosophila) |
| 别名 | Cancer/testis antigen 80, CT80, EC:3.1.26.-, HILI, Piwi like protein 2 |
| 计算分子量 | 110 kDa |
| 观测分子量 | 109 kDa |
| GenBank蛋白编号 | BC025995 |
| 基因名称 | PIWIL2 |
| Gene ID (NCBI) | 55124 |
| RRID | AB_3743179 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8TC59 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIWIL2 is a protein-coding gene that belongs to the Argonaute family of proteins, specifically the Piwi subfamily. The gene shows highly biased expression, found predominantly in the adult testis, with lower levels detected in the duodenum and other tissues. But PIWIL2 is widely expressed in a variety of tumors. PIWIL2 functions as an endoribonuclease ("slicer") that plays a central role in the piRNA (PIWI-interacting RNA) pathway . Its primary role is to protect the germline genome by repressing transposable elements (mobile genetic elements or "jumping genes"), thereby preventing their mobilization which could otherwise cause mutations and disrupt gametogenesis. (PMID: 35794855, PMID: 40789164)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PIWIL2 antibody 34244-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
