验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, HEK-293 cells, mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67201-1-Ig targets PITX2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, rat |
| 免疫原 |
CatNo: Ag27924 Product name: Recombinant human PITX2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-286 aa of BC013998 Sequence: MNCMKGPLHLEHRAAGTKLSAVSSSSCHHPQPLAMASVLAPGQPRSLDSSKHRLEVHTISDTSSPEAAEKDKSQQGKNEDVGAEDPSKKKRQRRQRTHFTSQQLQELEATFQRNRYPDMSTREEIAVWTNLTEARVRVWFKNRRAKWRKRERNQQAELCKNGFGPQFNGLMQPYDDMYPGYSYNNWAAKGLTSASLSTKSFPFFNSMNVNPLSSQSMFSPPNSISSMSMSSSMVPSAVTGVPGSSLNSLNNLNNLSSPSLNSAVPTPACPYAPPTPPYVYRDTCNS 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | paired-like homeodomain 2 |
| 别名 | 1E3B1, ALL1-responsive protein ARP1, ARP1, Paired-like homeodomain transcription factor 2, RIEG bicoid-related homeobox transcription factor |
| 计算分子量 | 36 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC013998 |
| 基因名称 | PITX2 |
| Gene ID (NCBI) | 5308 |
| RRID | AB_2882494 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q99697 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PITX2 belongs to the bicoid class of homeodomain transcription factors, which play essential roles in embryonic development. Various PITX2 isoforms are involved in a wide variety of developmental programs, including left-right signaling. It controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, PITX2 is involved in the expansion of muscle progenitors. In addition, it may play a role in the proper localization of asymmetric organs such as the heart and stomach. PITX2 can be bound by some other proteins, and are part of a much larger atrial transcriptional complex. This antibody specifically recognizes isoform PTX2C.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PITX2 antibody 67201-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oral Dis Enamel defects of Axenfeld-Rieger syndrome and the role of PITX2 in its pathogenesis.
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Aging (Albany NY) D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism
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Genomics Proteomics Bioinformatics Single-cell Atlas of Developing Mouse Palates Reveals Cellular and Molecular Transitions in Periderm Cell Fate |