验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, MCF-7 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human tonsillitis tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10873-1-AP targets PITX1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1303 Product name: Recombinant human PITX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-314 aa of BC009412 Sequence: MDAFKGGMSLERLPEGLRPPPPPPHDMGPAFHLARPADPREPLENSASESSDTELPEKERGGEPKGPEDSGAGGTGCGGADDPAKKKKQRRQRTHFTSQQLQELEATFQRNRYPDMSMREEIAVWTNLTEPRVRVWFKNRRAKWRKRERNQQLDLCKGGYVPQFSGLVQPYEDVYAAGYSYNNWAAKSLAPAPLSTKSFTFFNSMSPLSSQSMFSAPSSISSMTMPSSMGPGAVPGMPNSGLNNINNLTGSSLNSAMSPGACPYGTPASPYSVYRDTCNSSLASLRLKSKQHSSFGYGGLQGPASGLNACQYNS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | paired-like homeodomain 1 |
| 别名 | BFT, Hindlimb-expressed homeobox protein backfoot, Paired-like homeodomain transcription factor 1, POTX, PTX1 |
| 计算分子量 | 34 kDa |
| 观测分子量 | 38-40 kDa |
| GenBank蛋白编号 | BC009412 |
| 基因名称 | PITX1 |
| Gene ID (NCBI) | 5307 |
| RRID | AB_10859664 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P78337 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PITX1 (Paired-like homeodomain 1) is a bicoid-related homeobox transcription factor that plays critical roles in embryonic development, particularly in patterning the hindlimb and regulating pituitary organogenesis. It is also known as "Backfoot" due to its involvement in establishing left-right asymmetry during limb formation. Beyond development, PITX1 has been implicated in various cancers, where it can function as either a tumor suppressor or an oncogene depending on the cellular context, and its dysregulation is associated with tumor progression and metastasis.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PITX1 antibody 10873-1-AP | Download protocol |
| IP protocol for PITX1 antibody 10873-1-AP | Download protocol |
| WB protocol for PITX1 antibody 10873-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities. | ||
Hum Mutat Mandibular-pelvic-patellar syndrome is a novel PITX1-related disorder due to alteration of PITX1 transactivation ability. | ||
Endocrinology Proton Sensitivity of Corticotropin-Releasing Hormone Receptor 1 Signaling to Proopiomelanocortin in Male Mice. | ||
Int J Biochem Cell Biol Paired like homeodomain 1 and SAM and SH3 domain-containing 1 in the progression and prognosis of head and neck squamous cell carcinoma.
| ||
Toxicol Lett All-trans-retinoid acid (ATRA) may have inhibited chondrogenesis of primary hind limb bud mesenchymal cells by downregulating Pitx1 expression. |
