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  • Featured Product
  • KD/KO Validated

PITPNB Polyclonal antibody

PITPNB Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13110-1-AP

Print datasheet

Synonyms

PI TP beta, PITPNB, PtdIns transfer protein beta, PtdInsTP, PtdInsTP beta, VIB1B

验证数据展示

  • WB analysis using 13110-1-AP

    WB analysis using 13110-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13110-1-AP (PITPNB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 13110-1-AP

    IHC staining of human prostate cancer using 13110-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13110-1-AP (PITPNB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 13110-1-AP

    IHC staining of human prostate cancer using 13110-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13110-1-AP (PITPNB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, mouse lung tissue, HeLa cells, Raji cells, rat lung tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13110-1-AP targets PITPNB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PITPNB fusion protein Ag3774 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphatidylinositol transfer protein, beta
Synonyms PI TP beta, PITPNB, PtdIns transfer protein beta, PtdInsTP, PtdInsTP beta, VIB1B
Calculated Molecular Weight 272 aa, 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC031427
Gene Symbol PITPNB
Gene ID (NCBI) 23760
RRIDAB_2164999
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48739
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PITPNB antibody 13110-1-APDownload protocol
IHC protocol for PITPNB antibody 13110-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Virol

Nir2 Is an Effector of VAPs Necessary for Efficient Hepatitis C Virus Replication and Phosphatidylinositol 4-Phosphate Enrichment at the Viral Replication Organelle.

Authors - Hongliang Wang
  • KO Validated
View Article