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验证数据展示

  • WB analysis using 68571-1-Ig

    WB analysis using 68571-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68571-1-Ig (PIP5K3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68571-1-Ig

    WB analysis using 68571-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68571-1-Ig (PIP5K3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

PIP5K3 Monoclonal antibody

PIP5K3 Monoclonal Antibody for WB, ELISA
Cat No. 68571-1-Ig

产品说明书

CloneNo. 2F5G1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, ELISA

别名

PIKfyve, 1-phosphatidylinositol 3-phosphate 5-kinase, CFD, EC:2.7.1.150, EC:2.7.11.1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, HEK-293 cells, HepG2 cells, rat brain tissue, mouse brain tissue, rabbit brain tissue, pig brain tissue
Planning an IHC experiment? We recommend our IHCeasy® PIP5K3 Ready-To-Use IHC Kit. PIP5K3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68571-1-Ig targets PIP5K3 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag4366

Product name: Recombinant human PIP5K3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-451 aa of BC032389

Sequence: MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKGRR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III
别名 PIKfyve, 1-phosphatidylinositol 3-phosphate 5-kinase, CFD, EC:2.7.1.150, EC:2.7.11.1
计算分子量 451 aa, 50 kDa, 237 kDa
观测分子量 240-250 kDa
GenBank蛋白编号BC032389
基因名称 PIP5K3
Gene ID (NCBI) 200576
RRIDAB_3085272
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y2I7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIP5K3 antibody 68571-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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