验证数据展示

IHC staining of human liver cancer using 13361-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PIP5K3 antibody included
WB analysis using 13361-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 13361-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PIP5K3 antibody included
WB analysis using 13361-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of mouse liver using 13361-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PIP5K3 antibody included
IHC staining of human ovary cancer using 13361-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PIP5K3 antibody included
IHC staining of human placenta using 13361-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PIP5K3 antibody included
IHC staining of mouse ovary using 13361-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PIP5K3 antibody included
IHC staining of mouse thymus using 13361-1-AP
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PIP5K3 antibody included
IHC staining of rat liver using 13361-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PIP5K3 antibody included
IHC staining of rat ovary using 13361-1-AP
Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PIP5K3 antibody included

Featured Product
KD/KO Validated

PIP5K3 Polyclonal antibody

PIP5K3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13361-1-AP

产品说明书

引用文献(14)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, ELISA and More (1)

别名

PIKfyve, 1-phosphatidylinositol 3-phosphate 5-kinase, CFD, EC:2.7.1.150, EC:2.7.11.1

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (14)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, mouse lung tissue, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IHC detected in	human liver cancer tissue, mouse liver tissue, human ovary cancer tissue, rat liver tissue, mouse thymus tissue, rat ovary tissue, human placenta tissue, mouse ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Planning an IHC experiment? We recommend our IHCeasy® PIP5K3 Ready-To-Use IHC Kit. PIP5K3 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 6 publications below
IHC	See 3 publications below

产品信息

13361-1-AP targets PIP5K3 in WB, IHC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, ELISA Application Description
文献引用应用	WB, IHC, Dot Blot
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, pig, sheep
免疫原	CatNo: Ag4177 Product name: Recombinant human PIP5K3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-451 aa of BC032389 Sequence: MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKGRR 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III
别名	PIKfyve, 1-phosphatidylinositol 3-phosphate 5-kinase, CFD, EC:2.7.1.150, EC:2.7.11.1
计算分子量	451 aa, 50 kDa, 237 kDa
观测分子量	237 kDa
GenBank蛋白编号	BC032389
基因名称	PIP5K3
Gene ID (NCBI)	200576
RRID	AB_10638310
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9Y2I7
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

PIP5K3 (1-phosphatidylinositol 3-phosphate 5-kinase), also callled PIKFYVE, belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol (PMID: 15902656; 9858586). The content of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) in endosomal membranes changes dynamically with fission and fusion events that generate or absorb intracellular transport vesicles. PIKFYVE is the PtdIns(3,5)P2-producing component of a trimolecular complex that tightly regulates the level of PtdIns(3,5)P2. Other components of this complex are the PIKFYVE activator VAC14 and the PtdIns(3,5)P2 phosphatase FIG4 (PMID: 17556371). In addition to its phosphoinositide 5-kinase activity, PIKFYVE also has protein kinase activity (PubMed: 11714711; 17556371).PIP5K3 has four isoforms with molecular weights of 237 kDa, 50 kDa, 51 kDa and 62 kDa. This antibody primarily recognizes the 237 kDa isoform.

实验方案

Product Specific Protocols
IHC protocol for PIP5K3 antibody 13361-1-AP	Download protocol
WB protocol for PIP5K3 antibody 13361-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
		Cancer Cell A stress-adaptive lipid kinase axis defines metabolic vulnerabilities in neuroendocrine prostate cancer. Authors - Yang Zheng View Article
human	WB	Cell PIKFYVE inhibition mitigates disease in models of diverse forms of ALS Authors - Shu-Ting Hung View Article
human	WB	Autophagy Mitochondrial respiratory chain deficiency inhibits lysosomal hydrolysis. Authors - Lorena Fernandez-Mosquera View Article
		Mol Cell TSC1 binding to lysosomal PIPs is required for TSC complex translocation and mTORC1 regulation. Authors - Katharina Fitzian KD Validated View Article
human	IHC	Cell Rep Med Targeting the ferritinophagy-lysosome axis as a therapeutic vulnerability in gastroenteropancreatic neuroendocrine tumors Authors - Yizhi Cao View Article
human	WB	Acta Neuropathol Transient receptor potential vanilloid channel 2 contributes to multi-modal endoplasmic reticulum and perinuclear space dilations that can also be observed in prion-infected mice Authors - Wenda Zhao View Article

验证数据展示

IHC staining of human liver cancer using 13361-1-AP

WB analysis using 13361-1-AP

IHC staining of human liver cancer using 13361-1-AP

WB analysis using 13361-1-AP

IHC staining of mouse liver using 13361-1-AP

IHC staining of human ovary cancer using 13361-1-AP

IHC staining of human placenta using 13361-1-AP

IHC staining of mouse ovary using 13361-1-AP

IHC staining of mouse thymus using 13361-1-AP

IHC staining of rat liver using 13361-1-AP

IHC staining of rat ovary using 13361-1-AP

PIP5K3 Polyclonal antibody

PIP5K3 Polyclonal Antibody for WB, IHC, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

A stress-adaptive lipid kinase axis defines metabolic vulnerabilities in neuroendocrine prostate cancer.

PIKFYVE inhibition mitigates disease in models of diverse forms of ALS

Mitochondrial respiratory chain deficiency inhibits lysosomal hydrolysis.

TSC1 binding to lysosomal PIPs is required for TSC complex translocation and mTORC1 regulation.

Targeting the ferritinophagy-lysosome axis as a therapeutic vulnerability in gastroenteropancreatic neuroendocrine tumors

Transient receptor potential vanilloid channel 2 contributes to multi-modal endoplasmic reticulum and perinuclear space dilations that can also be observed in prion-infected mice

Able™

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