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验证数据展示

  • WB analysis using 83647-3-RR

    WB analysis using 83647-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83647-3-RR (PIP4K2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 83647-3-RR

    IHC staining of mouse brain using 83647-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83647-3-RR (PIP4K2B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIP4K2B antibody included
  • IHC staining of mouse brain using 83647-3-RR

    IHC staining of mouse brain using 83647-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83647-3-RR (PIP4K2B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIP4K2B antibody included
  • IF Staining of mouse brain using 83647-3-RR

    IF Staining of mouse brain using 83647-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PIP4K2B antibody (83647-3-RR, Clone: 240645F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of A549 using 83647-3-RR

    FC experiment of A549 using 83647-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PIP4K2B Recombinant antibody (83647-3-RR, Clone:240645F10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

PIP4K2B Recombinant monoclonal antibody

PIP4K2B Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 83647-3-RR

产品说明书

CloneNo. 240645F10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, FC (Intra), ELISA

别名

Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, EC:2.7.1.149, Diphosphoinositide kinase 2-beta, 240645F10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, A549 cells, PC-12 cells, NIH/3T3 cells, C2C12 cells, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy PIP4K2B Ready-To-Use IHC Kit. PIP4K2B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83647-3-RR targets PIP4K2B in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4099

Product name: Recombinant human PIP4K2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-281 aa of BC027459

Sequence: MSSNCTSTTAVAVAPLSASKTKTKKKHFVCQKVKLFRASEPILSVLMWGVNHTINELSNVPVPVMLMPDDFKAYSKIKVDNHLFNKENLPSRFKFKEYCPMVFRNLRERFGIDDQDYQNSVTRSAPINSDSQGRCGTRFLTTYDRRFVIKTVSSEDVAEMHNILKKYHQFIVECHGNTLLPQFLGMYRLTVDGVETYMVVTRNVFSHRLTVHRKYDLKGSTVAREASDKEKAKDLPTFKDNDFLNEGQKLHVGEESKKNFLEKLKRDVEEILVLSPGRRIA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphatidylinositol-5-phosphate 4-kinase, type II, beta
别名 Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, EC:2.7.1.149, Diphosphoinositide kinase 2-beta, 240645F10
计算分子量 416 aa, 47 kDa
观测分子量45 kDa
GenBank蛋白编号BC027459
基因名称 PIP4K2B
Gene ID (NCBI) 8396
RRIDAB_3671256
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP78356
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIP4K2B, also named as PIP5K2B, participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. PIP4K2B has a homodimer.

实验方案

Product Specific Protocols
FC protocol for PIP4K2B antibody 83647-3-RRDownload protocol
IF protocol for PIP4K2B antibody 83647-3-RRDownload protocol
IHC protocol for PIP4K2B antibody 83647-3-RRDownload protocol
WB protocol for PIP4K2B antibody 83647-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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