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验证数据展示

  • WB analysis using 83647-3-RR (same clone as 83647-3-PBS)

    WB analysis using 83647-3-RR (same clone as 83647-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83647-3-RR (PIP4K2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83647-3-RR (same clone as 83647-3-PBS)

    IHC staining of mouse brain using 83647-3-RR (same clone as 83647-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83647-3-RR (PIP4K2B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIP4K2B antibody included
  • IHC staining of mouse brain using 83647-3-RR (same clone as 83647-3-PBS)

    IHC staining of mouse brain using 83647-3-RR (same clone as 83647-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83647-3-RR (PIP4K2B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIP4K2B antibody included
  • IF Staining of mouse brain using 83647-3-RR (same clone as 83647-3-PBS)

    IF Staining of mouse brain using 83647-3-RR (same clone as 83647-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PIP4K2B antibody (83647-3-RR, Clone: 240645F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83647-3-RR (same clone as 83647-3-PBS)

    FC experiment of A549 using 83647-3-RR (same clone as 83647-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PIP4K2B Recombinant antibody (83647-3-RR, Clone:240645F10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

PIP4K2B Recombinant monoclonal antibody, PBS Only

PIP4K2B Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 83647-3-PBS

产品说明书

CloneNo. 240645F10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, FC (Intra), ELISA

别名

Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, EC:2.7.1.149, Diphosphoinositide kinase 2-beta, 240645F10

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PIP4K2B Ready-To-Use IHC Kit. PIP4K2B primary antibody included.

产品信息

83647-3-PBS targets PIP4K2B in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4099

Product name: Recombinant human PIP4K2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-281 aa of BC027459

Sequence: MSSNCTSTTAVAVAPLSASKTKTKKKHFVCQKVKLFRASEPILSVLMWGVNHTINELSNVPVPVMLMPDDFKAYSKIKVDNHLFNKENLPSRFKFKEYCPMVFRNLRERFGIDDQDYQNSVTRSAPINSDSQGRCGTRFLTTYDRRFVIKTVSSEDVAEMHNILKKYHQFIVECHGNTLLPQFLGMYRLTVDGVETYMVVTRNVFSHRLTVHRKYDLKGSTVAREASDKEKAKDLPTFKDNDFLNEGQKLHVGEESKKNFLEKLKRDVEEILVLSPGRRIA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphatidylinositol-5-phosphate 4-kinase, type II, beta
别名 Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, EC:2.7.1.149, Diphosphoinositide kinase 2-beta, 240645F10
计算分子量 416 aa, 47 kDa
观测分子量45 kDa
GenBank蛋白编号BC027459
基因名称 PIP4K2B
Gene ID (NCBI) 8396
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP78356
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PIP4K2B, also named as PIP5K2B, participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. PIP4K2B has a homodimer.

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