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验证数据展示

  • WB analysis using 23274-1-AP (same clone as 23274-1-PBS)

    WB analysis using 23274-1-AP (same clone as 23274-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

    IF Staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

  • WB analysis using 23274-1-AP (same clone as 23274-1-PBS)

    WB analysis using 23274-1-AP (same clone as 23274-1-PBS)

    Non-treated and CCCP treated HeLa cells were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

    IHC staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PINK1 antibody included
  • IHC staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

    IHC staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PINK1 antibody included
  • WB analysis of HeLa using 23274-1-AP (same clone as 23274-1-PBS)

    WB analysis of HeLa using 23274-1-AP (same clone as 23274-1-PBS)

    WB result of PINK1 antibody (23274-1-AP, 1:600) with si-Control and si-PINK1 transfected HeLa cells. This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PINK1 Polyclonal antibody, PBS Only

PINK1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 23274-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

BRPK, EC:2.7.11.1, PARK6, PTEN induced putative kinase 1, PTEN-induced putative kinase protein 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PINK1 Ready-To-Use IHC Kit. PINK1 primary antibody included.

产品信息

23274-1-PBS targets PINK1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19825

Product name: Recombinant human PINK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 336-581 aa of BC028215

Sequence: PRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PTEN induced putative kinase 1
别名 BRPK, EC:2.7.11.1, PARK6, PTEN induced putative kinase 1, PTEN-induced putative kinase protein 1
计算分子量 581 aa, 63 kDa
观测分子量 65 kDa, 45 kDa
GenBank蛋白编号BC028215
基因名称 PINK1
Gene ID (NCBI) 65018
RRIDAB_2879244
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9BXM7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half-life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.

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