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验证数据展示

  • WB analysis using 87952-1-RR (same clone as 87952-1-PBS)

    WB analysis using 87952-1-RR (same clone as 87952-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87952-1-RR (PIK3C3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87952-1-PBS in a different storage buffer formulation.

  • IP experiment of PC-3 using 87952-1-RR (same clone as 87952-1-PBS)

    IP experiment of PC-3 using 87952-1-RR (same clone as 87952-1-PBS)

    IP result of anti-VPS34 (IP:87952-1-RR, 4ug; Detection:87952-1-RR 1:800) with PC-3 cells lysate 960 ug. This data was developed using the same antibody clone with 87952-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 87952-1-RR (same clone as 87952-1-PBS)

    IHC staining of human stomach cancer using 87952-1-RR (same clone as 87952-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 87952-1-RR (VPS34 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87952-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIK3C3/VPS34 antibody included
  • IHC staining of human stomach cancer using 87952-1-RR (same clone as 87952-1-PBS)

    IHC staining of human stomach cancer using 87952-1-RR (same clone as 87952-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 87952-1-RR (VPS34 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87952-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIK3C3/VPS34 antibody included
  • Cytometric bead array sample test of MP03292-1

    Cytometric bead array sample test of MP03292-1

    The mean VPS34 concentration was determined to be 54.5 ng/mL in Hela cell extract based on a 1.2 mg/mL extract load, 15.6 ng/mL in PC-3 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP03292-1

    Cytometric bead array standard curve of MP03292-1

    Cytometric bead array standard curve of MP03292-1, VPS34 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87952-1-PBS. Detection antibody: 87952-2-PBS. Standard: Ag3110. Range: 0.312-40 ng/mL.

  • Affinity of 87952-1-RR

    Affinity of 87952-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87952-1-RR against Human VPS34 were performed. The affinity constant is 0.547 nM.

VPS34 Recombinant monoclonal antibody, PBS Only (Capture)

VPS34 Uni-rAb® Recombinant Antibody for WB, IHC, IP, Cytometric bead array, Indirect ELISA
Cat No. 87952-1-PBS

产品说明书

CloneNo. 260118B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Cytometric bead array, Indirect ELISA

别名

PIK3C3, EC:2.7.1.137, hVps34, PI 3 Kinase Class 3, PI 3-kinase p100

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PIK3C3/VPS34 Ready-To-Use IHC Kit. PIK3C3/VPS34 primary antibody included.

产品信息

87952-1-PBS targets VPS34 as part of a matched antibody pair:

MP03292-1: 87952-1-PBS capture and 87952-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3110

Product name: Recombinant human PIK3C3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 600-887 aa of BC033004

Sequence: KIRGIIPETATLFKSALMPAQLFFKTEDGGKYPVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSTKHGFMQFIQSVPVAEVLDTEGSIQNFFRKYAPSENGPNGISAEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLFHIDFGYILGRDPKPLPPPMKLNKEMVEGMGGTQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHYMQSLIDESVHALFAAVVEQIHKFAQYWRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphoinositide-3-kinase, class 3
别名 PIK3C3, EC:2.7.1.137, hVps34, PI 3 Kinase Class 3, PI 3-kinase p100
计算分子量 887 aa, 100 kDa
观测分子量 100 kDa
GenBank蛋白编号BC033004
基因名称 VPS34
Gene ID (NCBI) 5289
RRIDAB_3745836
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8NEB9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PIK3C3(Phosphatidylinositol 3-kinase catalytic subunit type 3) is also named as VPS34 and belongs to the PI3/PI4-kinase family.It is a catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes.It can form a dimer(PMID:20339072).

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