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验证数据展示

  • WB analysis using 24788-1-AP

    WB analysis using 24788-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24788-1-AP (PIK3C2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 24788-1-AP

    IP experiment of HeLa using 24788-1-AP

    IP result of anti-PIK3C2B (IP:24788-1-AP, 4ug; Detection:24788-1-AP 1:1500) with HeLa cells lysate 1240 ug.

  • IHC staining of human skeletal muscle using 24788-1-AP

    IHC staining of human skeletal muscle using 24788-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24788-1-AP (PIK3C2B Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skeletal muscle using 24788-1-AP

    IHC staining of human skeletal muscle using 24788-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24788-1-AP (PIK3C2B Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 24788-1-AP

    IF Staining of HeLa using 24788-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24788-1-AP (PIK3C2B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PIK3C2B Polyclonal antibody

PIK3C2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 24788-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

C2 PI3K, PI3K C2 beta, PIK3C2B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

24788-1-AP targets PIK3C2B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 PIK3C2B fusion protein Ag20474 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoinositide-3-kinase, class 2, beta polypeptide
别名 C2 PI3K, PI3K C2 beta, PIK3C2B
计算分子量 1634 aa, 185 kDa
观测分子量 160-185 kDa
GenBank蛋白编号BC132876
基因名称 PIK3C2B
Gene ID (NCBI) 5287
RRIDAB_2879725
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00750
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIK3C2B antibody 24788-1-APDownload protocol
IHC protocol for PIK3C2B antibody 24788-1-APDownload protocol
IF protocol for PIK3C2B antibody 24788-1-APDownload protocol
IP protocol for PIK3C2B antibody 24788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Anal Chem

Pseudotargeted MS Method for the Sensitive Analysis of Protein Phosphorylation in Protein Complexes.

Authors - Jiawen Lyu
View Article
humanWB

Cell Rep

ALKBH5 causes retinal pigment epithelium anomalies and choroidal neovascularization in age-related macular degeneration via the AKT/mTOR pathway

Authors - Ru-Xu Sun
View Article