验证数据展示
经过测试的应用
| Positive IP detected in | U-87 MG cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30623-1-AP targets PIGP in IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag29498 Product name: Recombinant human PIGP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 70-134 aa of BC005180 Sequence: YVLLFGINMMSTSPLDSIHTITDNYAKNQQQKKYQEEAIPALRDISISEVNQMFFLAAKELYTKN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphatidylinositol glycan anchor biosynthesis, class P |
| 别名 | Phosphatidylinositol N-acetylglucosaminyltransferase subunit P, NPD010, DSRC, DSCRC, DSCR5 |
| 计算分子量 | 18 kDa |
| 观测分子量 | 15~18 kDa |
| GenBank蛋白编号 | BC005180 |
| 基因名称 | PIGP |
| Gene ID (NCBI) | 51227 |
| RRID | AB_3669740 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P57054 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Phosphatidylinositol glycan anchor biosynthesis, class P (PIGP), a member of the large glycosylphosphatidylinositol (GPI) anchor biosynthesis gene family, encodes a subunit of the enzyme that catalyzes the first step of GPI anchor biosynthesis (PMID: 28334793). Mutations in the PIGP gene can lead to a variety of disorders, including developmental delay, intellectual disability, seizures, dysmorphic features, and diverse congenital anomalies.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for PIGP antibody 30623-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
