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CoraLite®594-conjugated PICALM Monoclonal antibody

PICALM Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F6G7

Cat No : CL594-67564

Print datasheet

Synonyms

CALM, CLTH, LAP, PICALM

验证数据展示

  • FC experiment of HEK-293 using CL594-67564

    FC experiment of HEK-293 using CL594-67564

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PICALM (CL594-67564, Clone:1F6G7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL594-67564

    IF Staining of HepG2 using CL594-67564

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 PICALM antibody (CL594-67564, Clone: 1F6G7 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 67564-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67564 targets PICALM in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, Mouse, Rat
Immunogen PICALM fusion protein Ag29512 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphatidylinositol binding clathrin assembly protein
Synonyms CALM, CLTH, LAP, PICALM
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC064357
Gene Symbol PICALM
Gene ID (NCBI) 8301
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13492
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS). (PMID: 33397371, PMID: 26075887, PMID: 26005850)

实验方案

Product Specific Protocols
IF protocol for CL594 PICALM antibody CL594-67564Download protocol
FC protocol for CL594 PICALM antibody CL594-67564Download protocol
Standard Protocols
Click here to view our Standard Protocols