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验证数据展示

  • WB analysis using 87344-1-RR

    WB analysis using 87344-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87344-1-RR (PICAL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of K-562 using 87344-1-RR

    IF Staining of K-562 using 87344-1-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using PICALM antibody (87344-1-RR, Clone: 252594B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87344-1-RR

    Affinity of 87344-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87344-1-RR against Human PICALM were performed. The affinity constant is 6.54 nM.

PICALM Recombinant monoclonal antibody

PICALM Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87344-1-RR

产品说明书

CloneNo. 252594B12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

CALM, CLTH, LAP, Phosphatidylinositol-binding clathrin assembly protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, Caco-2 cells, PC-3 cells, HepG2 cells, NIH/3T3 cells
Positive IF/ICC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy PICALM Ready-To-Use IHC Kit. PICALM primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87344-1-RR targets PICALM in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg5966

Product name: Recombinant human PICAL protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 2-652 aa of NM_007166.3

Sequence: SGQSLTDRITAAQHSVTGSAVSKTVCKATTHEIMGPKKKHLDYLIQCTNEMNVNIPQLADSLFERTTNSSWVVVFKSLITTHHLMVYGNERFIQYLASRNTLFNLSNFLDKSGLQGYDMSTFIRRYSRYLNEKAVSYRQVAFDFTKVKRGADGVMRTMNTEKLLKTVPIIQNQMDALLDFNVNSNELTNGVINAAFMLLFKDAIRLFAAYNEGIINLLEKYFDMKKNQCKEGLDIYKKFLTRMTRISEFLKVAEQVGIDRGDIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRLKELAKKPHTSLTTAASPVSTSAGGIMTAPAIDIFSTPSSSNSTSKLPNDLLDLQQPTFHPSVHPMSTASQVASTWGDPFSATVDAVDDAIPSLNPFLTKSSGDVHLSISSDVSTFTTRTPTHEMFVGFTPSPVAQPHPSAGLNVDFESVFGNKSTNVIVDSGGFDELGGLLKPTVASQNQNLPVAKLPPSKLVSDDLDSSLANLVGNLGIGNGTTKNDVNWSQPGEKKLTGGSNWQPKVAPTTAWNAATMAPPVMAYPATTPTGMIGYGIPPQMGSVPVMTQPTLIYSQPVMRPPNPFGPVSGAQIQFM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphatidylinositol binding clathrin assembly protein
别名 CALM, CLTH, LAP, Phosphatidylinositol-binding clathrin assembly protein
计算分子量71 kDa
观测分子量60-70 kDa
GenBank蛋白编号NM_007166.3
基因名称 PICALM
Gene ID (NCBI) 8301
RRIDAB_3745481
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13492-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS). (PMID: 33397371, PMID: 26075887, PMID: 26005850)

实验方案

Product Specific Protocols
IF protocol for PICALM antibody 87344-1-RRDownload protocol
WB protocol for PICALM antibody 87344-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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