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验证数据展示

  • WB analysis of mouse testis using 84189-4-RR

    WB analysis of mouse testis using 84189-4-RR

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84189-4-RR

    WB analysis using 84189-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84189-4-RR

    Affinity and Kinetic Characterization of 84189-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84189-4-RR against Human PIAS2 were performed. The affinity constant is 0.28 nM.

PIAS2 Recombinant antibody

PIAS2 Recombinant Antibody for WB, ELISA
Cat No. 84189-4-RR

产品说明书

CloneNo. 241474G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

E3 SUMO-protein ligase PIAS2, DIP, DAB2-interacting protein, ARIP3, Androgen receptor-interacting protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, mouse testis tissue, HeLa cells, HEK-293T cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84189-4-RR targets PIAS2 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 PIAS2 fusion protein Ag9032 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein inhibitor of activated STAT, 2
别名 E3 SUMO-protein ligase PIAS2, DIP, DAB2-interacting protein, ARIP3, Androgen receptor-interacting protein 3
计算分子量 63 kDa, 69 kDa
观测分子量63-68 kDa
GenBank蛋白编号BC015190
基因名称 PIAS2
Gene ID (NCBI) 9063
RRIDAB_3671742
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75928
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIAS2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. PIAS2 also plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. PIAS2 has 3 isoforms produced by alternative splicing with the MW of 68 kDa, 63 kDa and 45 kDa.

实验方案

Product Specific Protocols
WB protocol for PIAS2 antibody 84189-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols